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Regulation of MAPK Function by Direct Interaction with the Mating-Specific Gα in Yeast

Metodiev, Metodi V and Matheos, Dina and Rose, Mark D and Stone, David E (2002) 'Regulation of MAPK Function by Direct Interaction with the Mating-Specific Gα in Yeast.' Science, 296 (5572). pp. 1483-1486. ISSN 0036-8075

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<jats:p> The mating response of the budding yeast <jats:italic>Saccharomyces cerevisiae</jats:italic> is mediated by a prototypical heterotrimeric GTP-binding protein (G protein) and mitogen-activated protein kinase (MAPK) cascade. Although signal transmission by such pathways has been modeled in detail, postreceptor down-regulation is less well understood. The pheromone-responsive G protein α subunit (Gα) of yeast down-regulates the mating signal, but its targets are unknown. We have found that Gα binds directly to the mating-specific MAPK in yeast cells responding to pheromone. This interaction contributes both to modulation of the mating signal and to the chemotropic response, and it demonstrates direct communication between the top and bottom of a Gα-MAPK pathway. </jats:p>

Item Type: Article
Uncontrolled Keywords: Cell Nucleus; Cytoplasm; Saccharomyces cerevisiae; Heterotrimeric GTP-Binding Proteins; GTP-Binding Protein alpha Subunits; GTP-Binding Protein alpha Subunits, Gq-G11; Mitogen-Activated Protein Kinases; GTP-Binding Protein beta Subunits; Saccharomyces cerevisiae Proteins; Recombinant Fusion Proteins; Guanosine Diphosphate; Pheromones; MAP Kinase Signaling System; Down-Regulation; Amino Acid Sequence; Amino Acid Motifs; Phosphorylation; Mutation; Molecular Sequence Data
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
Q Science > QR Microbiology
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 17 Jul 2012 09:02
Last Modified: 18 Aug 2022 12:40

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