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Transcriptome analysis identifies a robust gene expression program in the mouse intestinal epithelium on aging

Kazakevych, Juri and Stoyanova, Elena and Liebert, Anke and Varga Weisz, Patrick (2019) 'Transcriptome analysis identifies a robust gene expression program in the mouse intestinal epithelium on aging.' Scientific Reports, 9 (1). ISSN 2045-2322

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Abstract

The intestinal epithelium undergoes constant regeneration driven by intestinal stem cells. How old age affects the transcriptome in this highly dynamic tissue is an important, but poorly explored question. Using transcriptomics on sorted intestinal stem cells and adult enterocytes, we identified candidate genes, which change expression on aging. Further validation of these on intestinal epithelium of multiple middle-aged versus old-aged mice highlighted the consistent up-regulation of the expression of the gene encoding chemokine receptor Ccr2, a mediator of inflammation and several disease processes. We observed also increased expression of Strc, coding for stereocilin, and dramatically decreased expression of Rps4l, coding for a ribosome subunit. Ccr2 and Rps4l are located close to the telomeric regions of chromosome 9 and 6, respectively. As only few genes were differentially expressed and we did not observe significant protein level changes of identified ageing markers, our analysis highlights the overall robustness of murine intestinal epithelium gene expression to old age.

Item Type: Article
Divisions: Faculty of Science and Health > Life Sciences, School of
Depositing User: Elements
Date Deposited: 15 Aug 2019 10:11
Last Modified: 15 Aug 2019 10:11
URI: http://repository.essex.ac.uk/id/eprint/25174

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