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Successful sample preparation for serial crystallography experiments

Beale, John H and Bolton, Rachel and Marshall, Stephen A and Beale, Emma V and Carr, Stephen B and Ebrahim, Ali and Moreno-Chicano, Tadeo and Hough, Michael A and Worrall, Jonathan AR and Tews, Ivo and Owen, Robin L (2019) 'Successful sample preparation for serial crystallography experiments.' Journal of Applied Crystallography, 52. 1385 - 1396. ISSN 0021-8898

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Abstract

Serial crystallography, at both synchrotron and X-ray free-electron laser light sources, is becoming increasingly popular. However, the tools in the majority of crystallization laboratories are focused on producing large single crystals by vapour diffusion that fit the cryo-cooled paradigm of modern synchrotron crystallography. This paper presents several case studies and some ideas and strategies on how to perform the conversion from a single crystal grown by vapour diffusion to the many thousands of micro-crystals required for modern serial crystallography grown by batch crystallization. These case studies aim to show (i) how vapour diffusion conditions can be converted into batch by optimizing the length of time crystals take to appear; (ii) how an understanding of the crystallization phase diagram can act as a guide when designing batch crystallization protocols; and (iii) an accessible methodology when attempting to scale batch conditions to larger volumes. These methods are needed to minimize the sample preparation gap between standard rotation crystallography and dedicated serial laboratories, ultimately making serial crystallography more accessible to all crystallographers.

Item Type: Article
Uncontrolled Keywords: serial macromolecular crystallography; XFELs; batch crystallization; vapour diffusion; micro-crystallization
Divisions: Faculty of Science and Health > Life Sciences, School of
Depositing User: Elements
Date Deposited: 21 Nov 2019 10:07
Last Modified: 21 Nov 2019 10:15
URI: http://repository.essex.ac.uk/id/eprint/25987

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