Research Repository

Reaction of O2 with a di-iron protein generates a mixed valent Fe2+/Fe3+ center and peroxide

Bradley, Justin and Svistunenko, Dimitri and Pullin, Jacob and Hill, Natalie and Stuart, Rhona and Palenik, Brian and Wilson, Michael and Hemmings, Andrew and Moore, Geoffrey and Le Brun, Nick (2019) 'Reaction of O2 with a di-iron protein generates a mixed valent Fe2+/Fe3+ center and peroxide.' Proceedings of the National Academy of Sciences, 116 (6). 2058 - 2067. ISSN 0027-8424

[img]
Preview
Text
SynFtn_manuscript_PNAS_accepted_version_with_figures.pdf - Accepted Version

Download (1MB) | Preview

Abstract

he gene encoding the cyanobacterial ferritin SynFtn is up-regulated in response to copper stress. Here, we show that, while SynFtn does not interact directly with copper, it is highly unusual in several ways. First, its catalytic diiron ferroxidase center is unlike those of all other characterized prokaryotic ferritins and instead resembles an animal H-chain ferritin center. Second, as demonstrated by kinetic, spectroscopic, and high-resolution X-ray crystallographic data, reaction of O2 with the di-Fe2+ center results in a direct, one-electron oxidation to a mixed-valent Fe2+/Fe3+ form. Iron–O2 chemistry of this type is currently unknown among the growing family of proteins that bind a diiron site within a four α-helical bundle in general and ferritins in particular. The mixed-valent form, which slowly oxidized to the more usual di-Fe3+ form, is an intermediate that is continually generated during mineralization. Peroxide, rather than superoxide, is shown to be the product of O2 reduction, implying that ferroxidase centers function in pairs via long-range electron transfer through the protein resulting in reduction of O2 bound at only one of the centers. We show that electron transfer is mediated by the transient formation of a radical on Tyr40, which lies ∼4 Å from the diiron center. As well as demonstrating an expansion of the iron–O2 chemistry known to occur in nature, these data are also highly relevant to the question of whether all ferritins mineralize iron via a common mechanism, providing unequivocal proof that they do not.

Item Type: Article
Uncontrolled Keywords: di-iron protein, ferritin, tyrosyl radical, iron
Divisions: Faculty of Science and Health > Life Sciences, School of
Depositing User: Elements
Date Deposited: 13 Feb 2020 18:46
Last Modified: 13 Feb 2020 18:46
URI: http://repository.essex.ac.uk/id/eprint/26430

Actions (login required)

View Item View Item