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RNA metabolism is the primary target of formamide in vivo

Hoyos-Manchado, Rafael and Reyes-Martín, Félix and Rallis, Charalampos and Gamero-Estévez, Enrique and Rodríguez-Gómez, Pablo and Quintero-Blanco, Juan and Bähler, Jürg and Jiménez, Juan and Tallada, Víctor A (2017) 'RNA metabolism is the primary target of formamide in vivo.' Scientific Reports, 7 (1). ISSN 2045-2322

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Abstract

The synthesis, processing and function of coding and non-coding RNA molecules and their interacting proteins has been the focus of a great deal of research that has boosted our understanding of key molecular pathways that underlie higher order events such as cell cycle control, development, innate immune response and the occurrence of genetic diseases. In this study, we have found that formamide preferentially weakens RNA related processes in vivo. Using a non-essential Schizosaccharomyces pombe gene deletion collection, we identify deleted loci that make cells sensitive to formamide. Sensitive deletions are significantly enriched in genes involved in RNA metabolism. Accordingly, we find that previously known temperature-sensitive splicing mutants become lethal in the presence of the drug under permissive temperature. Furthermore, in a wild type background, splicing efficiency is decreased and R-loop formation is increased in the presence of formamide. In addition, we have also isolated 35 formamide-sensitive mutants, many of which display remarkable morphology and cell cycle defects potentially unveiling new players in the regulation of these processes. We conclude that formamide preferentially targets RNA related processes in vivo, probably by relaxing RNA secondary structures and/or RNA-protein interactions, and can be used as an effective tool to characterize these processes.

Item Type: Article
Divisions: Faculty of Science and Health > Life Sciences, School of
Depositing User: Elements
Date Deposited: 18 Jun 2021 15:32
Last Modified: 18 Jun 2021 16:15
URI: http://repository.essex.ac.uk/id/eprint/26693

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