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Cell-based screens and phenomics with fission yeast.

Rallis, Charalampos and Bähler, Jürg (2016) 'Cell-based screens and phenomics with fission yeast.' Critical Reviews in Biochemistry and Molecular Biology, 51 (2). pp. 86-95. ISSN 1040-9238

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Next-generation sequencing approaches have considerably advanced our understanding of genome function and regulation. However, the knowledge of gene function and complex cellular processes remains a challenge and bottleneck in biological research. Phenomics is a rapidly emerging area, which seeks to rigorously characterize all phenotypes associated with genes or gene variants. Such high-throughput phenotyping under different conditions can be a potent approach toward gene function. The fission yeast Schizosaccharomyces pombe (S. pombe) is a proven eukaryotic model organism that is increasingly used for genomewide screens and phenomic assays. In this review, we highlight current large-scale, cell-based approaches used with S. pombe, including computational colony-growth measurements, genetic interaction screens, parallel profiling using barcodes, microscopy-based cell profiling, metabolomic methods and transposon mutagenesis. These diverse methods are starting to offer rich insights into the relationship between genotypes and phenotypes.

Item Type: Article
Uncontrolled Keywords: Schizosaccharomyces; Amino Acids; DNA Transposable Elements; Mutagenesis; Phenotype; Genome, Viral; Models, Biological; Metabolomics
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 18 Jun 2021 11:41
Last Modified: 18 Aug 2022 10:32

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