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Thermography methods to assess stomatal behaviour in a dynamic environment.

Vialet-Chabrand, Silvere and Lawson, Tracy (2020) 'Thermography methods to assess stomatal behaviour in a dynamic environment.' Journal of Experimental Botany, 71 (7). 2329 - 2338. ISSN 0022-0957

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Abstract

Although thermography allows rapid, non-invasive measurements of large numbers of plants, it has not been used extensively due to the difficulty in deriving biologically relevant information such as leaf transpiration (E) and stomatal conductance (gsw) from thermograms. Methods normalizing leaf temperature using temperatures from reference materials (e.g. with and without evaporative flux) to generate stress indices are generally preferred due to their ease of use to assess plant water status. Here, a simplified method to solve dynamic energy balance equations is presented, which enables the calculation of 'wet' and 'dry' leaf temperatures in order to derive stress indices, whilst providing accurate estimates of E and gsw. Comparing stress indices and gas exchange parameters highlights the limitation of stress indices in a dynamic environment and how this problem can be overcome using artificial leaf references with known conductance. Additionally, applying the equations for each pixel of a thermogram to derive the rapidity of stomatal response over the leaf lamina in wheat revealed the spatial heterogeneity of stomatal behaviour. Rapidity of stomatal movements is an important determinant of water use efficiency, and our results showed 'patchy' responses that were linked to both the spatial and temporal response of gsw.

Item Type: Article
Uncontrolled Keywords: Energy balance, patchy response, stomatal conductance, stress indices, thermal imaging, transpiration
Divisions: Faculty of Science and Health > Life Sciences, School of
Depositing User: Elements
Date Deposited: 21 Apr 2020 11:08
Last Modified: 21 Apr 2020 11:08
URI: http://repository.essex.ac.uk/id/eprint/27345

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