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Constitutively elevated levels of SOCS1 suppress innate responses in DF-1 immortalised chicken fibroblast cells.

Giotis, ES and Ross, CS and Robey, RC and Nohturfft, A and Goodbourn, S and Skinner, MA (2017) 'Constitutively elevated levels of SOCS1 suppress innate responses in DF-1 immortalised chicken fibroblast cells.' Scientific Reports, 7 (1). ISSN 2045-2322

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Abstract

The spontaneously immortalised DF-1 cell line is rapidly replacing its progenitor primary chicken embryo fibroblasts (CEFs) for studies on avian viruses such as avian influenza but no comprehensive study has as yet been reported comparing their innate immunity phenotypes. We conducted microarray analyses of DF-1 and CEFs, under both normal and stimulated conditions using chicken interferon-α (chIFN-α) and the attenuated infectious bursal disease virus vaccine strain PBG98. We found that DF-1 have an attenuated innate response compared to CEFs. Basal expression levels of Suppressor of Cytokine Signalling 1 (chSOCS1), a negative regulator of cytokine signalling in mammals, are 16-fold higher in DF-1 than in CEFs. The chSOCS1 "SOCS box" domain (which in mammals, interacts with an E3 ubiquitin ligase complex) is not essential for the inhibition of cytokine-induced JAK/STAT signalling activation in DF-1. Overexpression of SOCS1 in chIFN-α-stimulated DF-1 led to a relative decrease in expression of interferon-stimulated genes (ISGs; MX1 and IFIT5) and increased viral yield in response to PBG98 infection. Conversely, knockdown of SOCS1 enhanced induction of ISGs and reduced viral yield in chIFN-α-stimulated DF-1. Consequently, SOCS1 reduces induction of the IFN signalling pathway in chicken cells and can potentiate virus replication.

Item Type: Article
Uncontrolled Keywords: Cell Line, Fibroblasts, Animals, Chickens, Infectious bursal disease virus, Birnaviridae Infections, Bird Diseases, Avian Proteins, Interferon-alpha, Microarray Analysis, Cell Proliferation, Gene Expression, STAT Transcription Factors, Janus Kinases, Immunity, Innate, Gene Knockdown Techniques, Primary Cell Culture, Suppressor of Cytokine Signaling 1 Protein
Divisions: Faculty of Science and Health > Life Sciences, School of
Depositing User: Elements
Date Deposited: 06 Jun 2020 07:57
Last Modified: 06 Jun 2020 08:15
URI: http://repository.essex.ac.uk/id/eprint/27819

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