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Chicken Embryonic-Stem Cells Are Permissive to Poxvirus Recombinant Vaccine Vectors.

Giotis, Efstathios S and Montillet, Guillaume and Pain, Bertrand and Skinner, Michael A (2019) 'Chicken Embryonic-Stem Cells Are Permissive to Poxvirus Recombinant Vaccine Vectors.' Genes, 10 (3). ISSN 2073-4425

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Abstract

The discovery of mammalian pluripotent embryonic stem cells (ESC) has revolutionised cell research and regenerative medicine. More recently discovered chicken ESC (cESC), though less intensively studied, are increasingly popular as vaccine substrates due to a dearth of avian cell lines. Information on the comparative performance of cESC with common vaccine viruses is limited. Using RNA-sequencing, we compared cESC transcriptional programmes elicited by stimulation with chicken type I interferon or infection with vaccine viruses routinely propagated in primary chicken embryo fibroblasts (CEF). We used poxviruses (fowlpox virus (FWPV) FP9, canarypox virus (CNPV), and modified vaccinia virus Ankara (MVA)) and a birnavirus (infectious bursal disease virus (IBDV) PBG98). Interferon-stimulated genes (ISGs) were induced in cESC to levels comparable to those in CEF and immortalised chicken fibroblast DF-1 cells. cESC are permissive (with distinct host transcriptional responses) to MVA, FP9, and CNPV but, surprisingly, not to PBG98. MVA, CNPV, and FP9 suppressed innate immune responses, while PBG98 induced a subset of ISGs. Dysregulation of signalling pathways (i.e., NFκB, TRAF) was observed, which might affect immune responses and viral replication. In conclusion, we show that cESC are an attractive alternative substrate to study and propagate poxvirus recombinant vaccine vectors.

Item Type: Article
Uncontrolled Keywords: Cells, Cultured, Chick Embryo, Animals, Poxviridae, Interferon Type I, Viral Vaccines, Gene Expression Profiling, Sequence Analysis, RNA, Species Specificity, Gene Expression Regulation, Embryonic Stem Cells, Gene Regulatory Networks
Divisions: Faculty of Science and Health > Life Sciences, School of
Depositing User: Elements
Date Deposited: 06 Jun 2020 07:49
Last Modified: 06 Jun 2020 08:15
URI: http://repository.essex.ac.uk/id/eprint/27820

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