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Knockdown of CD-74 in the Proliferative and Apoptotic Activity of Breast Cancer Cells.

Ssadh, Hussain Al and Abdulmonem, Waleed Al and Rasheed, Zafar and Madar, Inamul Hasan and Alhoderi, Jamila and Eldeen, Samah K Nasr and Alradhwan, Ali and Alasmael, Noura and Alkhamiss, Abdullah and Fernández, Nelson (2019) 'Knockdown of CD-74 in the Proliferative and Apoptotic Activity of Breast Cancer Cells.' Open Access Macedonian Journal of Medical Sciences, 7 (19). pp. 3169-3176. ISSN 1857-9655

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BACKGROUND:The cluster of differentiation (CD) 74 is known for its immunological functions and its elevated level was reported in various cancer cells. AIM:The aim of the present study was to investigate the expression and potential roles of CD74 in the proliferative and apoptotic activity of breast cancer. METHODS:Expression of CD74, macrophage migration inhibitory factor (MIF) and CD44 was assayed in CAMA-1 and MDA-MB-231 cell lines using flow cytometry. CD74 was knocked down using CD74 siRNA-transfection in CAMA-1, and MDA-MB-231 cells and proliferation and apoptosis were determined in the transfected breast cancer cells. RESULTS:The data showed that CD74, MIF and CD44 were expressed in breast cancer cell lines and were associated with cell proliferation and apoptosis. Correlation analysis revealed that CD74 was positively correlated and colocalised with MIF on the cell-surface of CAMA-1 and MDA-MB-231. The knockdown of CD74 significantly reduced CAMA-1 and MDA-MB-231 cell proliferation and increased the level of apoptotic cells. CONCLUSION:We concluded that the interactions of CD74 with MIF and CD74 with CD44 could be a potential tumour marker for breast cancer cells. Moreover, the level of co-expression of MIF and CD74 or CD44 could be a surrogate marker for the efficacy of anti-angiogenic drugs, particularly in breast cancer tumours. In short, the study revealed the potential roles of CD74 in the proliferation and apoptosis of breast cancer which may serve as a potential therapeutic target for breast cancer.

Item Type: Article
Uncontrolled Keywords: Apoptosis; Breast cancer; CAMA-1; CD 74; Knockdown; MDA-MB-231 cells; Proliferation
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 11 Jan 2021 11:59
Last Modified: 06 Jan 2022 14:09

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