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Bespoke Biomolecular Wires for Transmembrane Electron Transfer: Spontaneous Assembly of a Functionalized Multiheme Electron Conduit

Piper, Samuel EH and Edwards, Marcus J and van Wonderen, Jessica H and Casadevall, Carla and Martel, Anne and Jeuken, Lars JC and Reisner, Erwin and Clarke, Thomas A and Butt, Julea N (2021) 'Bespoke Biomolecular Wires for Transmembrane Electron Transfer: Spontaneous Assembly of a Functionalized Multiheme Electron Conduit.' Frontiers in Microbiology, 12. 714508-. ISSN 1664-302X

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Abstract

Shewanella oneidensis exchanges electrons between cellular metabolism and external redox partners in a process that attracts much attention for production of green electricity (microbial fuel cells) and chemicals (microbial electrosynthesis). A critical component of this pathway is the outer membrane spanning MTR complex, a biomolecular wire formed of the MtrA, MtrB, and MtrC proteins. MtrA and MtrC are decaheme cytochromes that form a chain of close-packed hemes to define an electron transfer pathway of 185 Å. MtrA is wrapped inside MtrB for solubility across the outer membrane lipid bilayer; MtrC sits outside the cell for electron exchange with external redox partners. Here, we demonstrate tight and spontaneous <jats:italic>in vitro</jats:italic> association of MtrAB with separately purified MtrC. The resulting complex is comparable with the MTR complex naturally assembled by <jats:italic>Shewanella</jats:italic> in terms of both its structure and rates of electron transfer across a lipid bilayer. Our findings reveal the potential for building bespoke electron conduits where MtrAB combines with chemically modified MtrC, in this case, labeled with a Ru-dye that enables light-triggered electron injection into the MtrC heme chain.

Item Type: Article
Uncontrolled Keywords: cytochrome; electron transfer; microbial fuel cell; microbial electrosynthesis; photosensitizer; biomolecular wire; SANS; Shewanella
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 13 Oct 2021 10:12
Last Modified: 18 Aug 2022 10:48
URI: http://repository.essex.ac.uk/id/eprint/30982

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