Research Repository

Stomatal Responses to Light, CO2, and Mesophyll Tissue in Vicia faba and Kalanchoë fedtschenkoi

Santos, Mauro G and Davey, Phillip A and Hofmann, Tanja A and Borland, Anne and Hartwell, James and Lawson, Tracy (2021) 'Stomatal Responses to Light, CO2, and Mesophyll Tissue in Vicia faba and Kalanchoë fedtschenkoi.' Frontiers in Plant Science, 12. 740534-. ISSN 1664-462X

Santos et al 2021.pdf - Published Version
Available under License Creative Commons Attribution.

Download (6MB) | Preview


The responses of stomatal aperture to light intensity and CO₂ concentration were studied in both Vicia faba (C₃) and Kalanchoë fedtschenkoi (Crassulacean acid metabolism; CAM), in material sampled from both light and dark periods. Direct comparison was made between intact leaf segments, epidermises grafted onto exposed mesophyll, and isolated epidermal peels, including transplantations between species and between diel periods. We reported the stomatal opening in response to darkness in isolated CAM peels from the light period, but not from the dark. Furthermore, we showed that C₃ mesophyll has stimulated CAM stomata in transplanted peels to behave as C₃ in response to light and CO₂. By using peels and mesophyll from plants sampled in the dark and the light period, we provided clear evidence that CAM stomata behaved differently from C3. This might be linked to stored metabolites/ions and signalling pathway components within the guard cells, and/or a mesophyll-derived signal. Overall, our results provided evidence for both the involvement of guard cell metabolism and mesophyll signals in stomatal responses in both C₃ and CAM species.

Item Type: Article
Uncontrolled Keywords: stomata; CAM; mesophyll; Vicia faba; Kalanchoë fedtschenkoi; conductance; stomatal dynamics
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 26 Nov 2021 15:26
Last Modified: 06 Jan 2022 14:33

Actions (login required)

View Item View Item