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Microfluidic-Based Bacterial Molecular Computing on a Chip

Martins, Daniel P and Barros, Michael Taynnan and O'Sullivan, Benjamin J and Seymour, Ian and O'Riordan, Alan and Coffey, Lee and Sweeney, Joseph B and Balasubramaniam, Sasitharan (2022) 'Microfluidic-Based Bacterial Molecular Computing on a Chip.' IEEE Sensors Journal, 22 (17). pp. 16772-16784. ISSN 1530-437X

Microfluidic-based_Bacterial_Molecular_Computing_on_a_Chip.pdf - Accepted Version

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Biocomputing systems based on engineered bacteria can lead to novel tools for environmental monitoring and detection of metabolic diseases. In this paper, we propose a Bacterial Molecular Computing on a Chip (BMCoC) using microfluidic and electrochemical sensing technologies. The computing can be flexibly integrated into the chip, but we focus on engineered bacterial AND Boolean logic gate and ON-OFF switch sensors that produces secondary signals to change the pH and dissolved oxygen concentrations. We present a prototype with experimental results that shows the electrochemical sensors can detect small pH and dissolved oxygen concentration changes created by the engineered bacterial populations’ molecular signals. Additionally, we present a theoretical model analysis of the BMCoC computation reliability when subjected to unwanted effects, i.e., molecular signal delays and noise, and electrochemical sensors threshold settings that are based on either standard or blind detectors. Our numerical analysis found that the variations in the production delay and the molecular output signal concentration can impact on the computation reliability for the AND logic gate and ON-OFF switch. The molecular communications of synthetic engineered cells for logic gates integrated with sensing systems can lead to a new breed of biochips that can be used for numerous diagnostic applications.

Item Type: Article
Uncontrolled Keywords: Bacterial molecular computing; biosensors; electrochemical sensing; microfluidics; molecular communications; synthetic logic gates
Divisions: Faculty of Science and Health > Computer Science and Electronic Engineering, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 11 Oct 2022 18:43
Last Modified: 11 Oct 2022 18:43

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