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Full-length transcript sequencing of human and mouse cerebral cortex identifies widespread isoform diversity and alternative splicing.

Leung, Szi Kay and Jeffries, Aaron R and Castanho, Isabel and Jordan, Ben T and Moore, Karen and Davies, Jonathan P and Dempster, Emma L and Bray, Nicholas J and O'Neill, Paul and Tseng, Elizabeth and Ahmed, Zeshan and Collier, David A and Jeffery, Erin D and Prabhakar, Shyam and Schalkwyk, Leonard and Jops, Connor and Gandal, Michael J and Sheynkman, Gloria M and Hannon, Eilis and Mill, Jonathan (2021) 'Full-length transcript sequencing of human and mouse cerebral cortex identifies widespread isoform diversity and alternative splicing.' Cell Reports, 37 (7). p. 110022. ISSN 2211-1247

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Abstract

Alternative splicing is a post-transcriptional regulatory mechanism producing distinct mRNA molecules from a single pre-mRNA with a prominent role in the development and function of the central nervous system. We used long-read isoform sequencing to generate full-length transcript sequences in the human and mouse cortex. We identify novel transcripts not present in existing genome annotations, including transcripts mapping to putative novel (unannotated) genes and fusion transcripts incorporating exons from multiple genes. Global patterns of transcript diversity are similar between human and mouse cortex, although certain genes are characterized by striking differences between species. We also identify developmental changes in alternative splicing, with differential transcript usage between human fetal and adult cortex. Our data confirm the importance of alternative splicing in the cortex, dramatically increasing transcriptional diversity and representing an important mechanism underpinning gene regulation in the brain. We provide transcript-level data for human and mouse cortex as a resource to the scientific community.

Item Type: Article
Uncontrolled Keywords: Alternative Splicing; Animals; Brain; Cerebral Cortex; Exons; Gene Expression; Gene Expression Profiling; Genome; High-Throughput Nucleotide Sequencing; Humans; Mice; Protein Isoforms; RNA Precursors; RNA Splice Sites; RNA, Messenger; Sequence Analysis, RNA; Transcriptome
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 25 Sep 2022 15:32
Last Modified: 25 Sep 2022 15:32
URI: http://repository.essex.ac.uk/id/eprint/33553

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