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Dynamics of Lesion Processing by Bacterial Nucleotide Excision Repair Proteins

Kad, Neil M and Van Houten, Bennett (2012) 'Dynamics of Lesion Processing by Bacterial Nucleotide Excision Repair Proteins.' In: Doetsch, Paul W, (ed.) Progress in Molecular Biology and Translational Science. Elsevier, pp. 1-24. ISBN 9780123876652

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Single-molecule approaches permit an unrivalled view of how complex systems operate and have recently been used to understand DNA–protein interactions. These tools have enabled advances in a particularly challenging problem, the search for damaged sites on DNA. DNA repair proteins are present at the level of just a few hundred copies in bacterial cells to just a few thousand in human cells, and they scan the entire genome in search of their specific substrates. How do these proteins achieve this herculean task when their targets may differ from undamaged DNA by only a single hydrogen bond? Here we examine, using single-molecule approaches, how the prokaryotic nucleotide excision repair system balances the necessity for speed against specificity. We discuss issues at a theoretical, biological, and technical level and finally pose questions for future research.

Item Type: Book Section
Additional Information: Bacterial nucleotide excision repair; UvrA; UvrB; UvrC; Single molecule; DNA sliding; DNA hopping; DNA jumping; DNA damage recognition
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health > Biological Sciences, School of
Depositing User: Neil Kad
Date Deposited: 23 Jan 2013 12:17
Last Modified: 23 Jan 2013 12:17

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