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Physico-chemical foundations underpinning microarray and next-generation sequencing experiments

Harrison, A and Binder, H and Buhot, A and Burden, CJ and Carlon, E and Gibas, C and Gamble, LJ and Halperin, A and Hooyberghs, J and Kreil, DP and Levicky, R and Noble, PA and Ott, A and Pettitt, BM and Tautz, D and Pozhitkov, AE (2013) 'Physico-chemical foundations underpinning microarray and next-generation sequencing experiments.' Nucleic Acids Research, 41 (5). 2779 - 2796. ISSN 0305-1048

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Abstract

Hybridization of nucleic acids on solid surfaces is a key process involved in high-throughput technologies such as microarrays and, in some cases, nextgeneration sequencing (NGS). A physical understanding of the hybridization process helps to determine the accuracy of these technologies. The goal of a widespread research program is to develop reliable transformations between the raw signals reported by the technologies and individual molecular concentrations from an ensemble of nucleic acids. This research has inputs from many areas, from bioinformatics and biostatistics, to theoretical and experimental biochemistry and biophysics, to computer simulations. A group of leading researchers met in Ploen Germany in 2011 to discuss present knowledge and limitations of our physico-chemical understanding of high-throughput nucleic acid technologies. This meeting inspired us to write this summary, which provides an overview of the state-of-the-art approaches based on physicochemical foundation to modeling of the nucleic acids hybridization process on solid surfaces. In addition, practical application of current knowledge is emphasized. © The Author(s) 2013.

Item Type: Article
Subjects: Q Science > Q Science (General)
Divisions: Faculty of Science and Health > Mathematical Sciences, Department of
Depositing User: Andrew Harrison
Date Deposited: 02 Feb 2013 17:27
Last Modified: 23 Jan 2019 00:18
URI: http://repository.essex.ac.uk/id/eprint/5416

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