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In vitro suppression of drug-induced methaemoglobin formation by Intralipid� in whole human blood: observations relevant to the ?lipid sink theory?

Samuels, TL and Willers, JW and Uncles, DR and Monteiro, R and Halloran, C and Dai, H (2011) 'In vitro suppression of drug-induced methaemoglobin formation by Intralipid� in whole human blood: observations relevant to the ?lipid sink theory?' Anaesthesia, 67 (1). pp. 23-32. ISSN 0003-2409

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Abstract

To provide further evidence for the lipid sink theory, we have developed an in vitro model to assess the effect of Intralipid� 20% on methaemoglobin formation by drugs of varying lipid solubility. Progressively increasing Intralipid concentrations from 4 to 24 mg.ml?1 suppressed methaemoglobin formation by the lipid soluble drug glyceryl trinitrate in a dose-dependent manner (p < 0.001). Both dose and timing of administration of Intralipid to blood previously incubated with glyceryl trinitrate for 10 and 40 min resulted in significant suppression of methaemoglobin formation (p < 0.0001 and p < 0.05, respectively). Mathematical modelling demonstrated that the entire process of methaemoglobin formation by glyceryl trinitrate was slowed down in the presence of Intralipid. Intralipid did not significantly suppress methaemoglobin formation induced by 2-amino-5-hydroxytoluene (partially lipid soluble) or sodium nitrite (lipid insoluble; both p > 0.5). This work may assist determination of the suitability of drugs taken in overdose for which Intralipid might be deployed.

Item Type: Article
Subjects: Q Science > QA Mathematics
R Medicine > R Medicine (General)
Divisions: Faculty of Science and Health
Faculty of Science and Health > Mathematical Sciences, Department of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 12 Feb 2013 07:55
Last Modified: 06 Jan 2022 13:40
URI: http://repository.essex.ac.uk/id/eprint/5497

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