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Structural and Regulatory Genes Required to Make the Gas Dimethyl Sulfide in Bacteria

Todd, Jonathan D and Rogers, Rachel and Li, You Guo and Wexler, Margaret and Bond, Philip L and Sun, Lei and Curson, Andrew RJ and Malin, Gill and Steinke, Michael and Johnston, Andrew WB (2007) 'Structural and Regulatory Genes Required to Make the Gas Dimethyl Sulfide in Bacteria.' Science, 315 (5812). pp. 666-669. ISSN 0036-8075

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Abstract

<jats:p> Dimethyl sulfide (DMS) is a key compound in global sulfur and carbon cycles. DMS oxidation products cause cloud nucleation and may affect weather and climate. DMS is generated largely by bacterial catabolism of dimethylsulfoniopropionate (DMSP), a secondary metabolite made by marine algae. We demonstrate that the bacterial gene <jats:italic>dddD</jats:italic> is required for this process and that its transcription is induced by the DMSP substrate. Cloned <jats:italic>dddD</jats:italic> from the marine bacterium <jats:italic>Marinomonas</jats:italic> and from two bacterial strains that associate with higher plants, the N <jats:sub>2</jats:sub> -fixing symbiont <jats:italic>Rhizobium</jats:italic> NGR234 and the root-colonizing <jats:italic>Burkholderia cepacia</jats:italic> AMMD, conferred to <jats:italic>Escherichia coli</jats:italic> the ability to make DMS from DMSP. The inferred enzymatic mechanism for DMS liberation involves an initial step in which DMSP is modified by addition of acyl coenzyme A, rather than the immediate release of DMS by a DMSP lyase, the previously suggested mechanism. </jats:p>

Item Type: Article
Uncontrolled Keywords: Burkholderia cepacia; Rhizobium; Escherichia coli; Poaceae; Sulfides; Sulfonium Compounds; Coenzyme A-Transferases; Bacterial Proteins; DNA Transposable Elements; Cloning, Molecular; Transformation, Bacterial; Amino Acid Sequence; Oxidation-Reduction; Phenotype; Genes, Bacterial; Operon; Genes, Regulator; Molecular Sequence Data; Marinomonas; Promoter Regions, Genetic
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 07 Mar 2013 14:54
Last Modified: 18 Aug 2022 13:01
URI: http://repository.essex.ac.uk/id/eprint/5661

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