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Cultivation of Halophilic Hydrocarbon Degraders

McGenity, TJ and Gramain, A (2010) 'Cultivation of Halophilic Hydrocarbon Degraders.' In: Timmis, KN and McGenity, TJ and van der Meer, JR and de Lorenzo, V, (eds.) Handbook of Hydrocarbon and Lipid Microbiology. Springer, 3847 - 3854. ISBN 9783540775843

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Abstract

Few extremely halophilic hydrocarbon-degrading microbes have been grown in pure culture, and so in this chapter we outline some of the strategies that have been successfully adopted, with primary consideration to enhancing the bioavailability of hydrocarbons at high salinity. A minimal medium is presented, but it should be considered only as a starting point, and adapted to reflect the salt composition of the environment. General strategies for growing previously uncultivated microbes are briefly reviewed, and the approaches used to add hydrocarbons to media are also considered. In order to increase the chances of cultivating halophilic hydrocarbon degraders, we suggest that the growth medium is prepared or supplemented with brine from the environment under study.

Item Type: Book Section
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Science and Health > Life Sciences, School of
Depositing User: Terence McGenity
Date Deposited: 24 Apr 2013 09:48
Last Modified: 25 Sep 2019 10:16
URI: http://repository.essex.ac.uk/id/eprint/5934

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