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The role of proteases in the differentiation of Acanthamoeba castellanii.

Dudley, R and Alsam, S and Khan, NA (2008) 'The role of proteases in the differentiation of Acanthamoeba castellanii.' FEMS microbiology letters, 286 (1). 9 - 15. ISSN 0378-1097

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Abstract

Proteases are significant determinants of protozoan pathogenicity and cytolysis of host cells. However, there is now growing evidence of their involvement in cellular differentiation. Acanthamoeba castellanii of the T4 genotype elaborates a number of proteases, which are inhibited by the serine protease inhibitor phenylmethylsulphonyl fluoride. Using this and other selective protease inhibitors, in tandem with siRNA primers, specific to the catalytic site of Acanthamoeba serine proteases, we demonstrate that serine protease activity is crucial for the differentiation of A. castellanii. Furthermore, both encystment and excystment of A. castellanii was found to be dependent on serine protease function.

Item Type: Article
Uncontrolled Keywords: Acanthamoeba; differentiation; serine proteases
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health > Life Sciences, School of
Depositing User: Jim Jamieson
Date Deposited: 16 Sep 2011 09:02
Last Modified: 17 Oct 2019 14:17
URI: http://repository.essex.ac.uk/id/eprint/675

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