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Chlorophyll fluorescence: a probe of photosynthesis in vivo.

Baker, NR (2008) 'Chlorophyll fluorescence: a probe of photosynthesis in vivo.' Annual review of plant biology, 59 (1). pp. 89-113. ISSN 1543-5008

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The use of chlorophyll fluorescence to monitor photosynthetic performance in algae and plants is now widespread. This review examines how fluorescence parameters can be used to evaluate changes in photosystem II (PSII) photochemistry, linear electron flux, and CO(2) assimilation in vivo, and outlines the theoretical bases for the use of specific fluorescence parameters. Although fluorescence parameters can be measured easily, many potential problems may arise when they are applied to predict changes in photosynthetic performance. In particular, consideration is given to problems associated with accurate estimation of the PSII operating efficiency measured by fluorescence and its relationship with the rates of linear electron flux and CO(2) assimilation. The roles of photochemical and nonphotochemical quenching in the determination of changes in PSII operating efficiency are examined. Finally, applications of fluorescence imaging to studies of photosynthetic heterogeneity and the rapid screening of large numbers of plants for perturbations in photosynthesis and associated metabolism are considered.

Item Type: Article
Uncontrolled Keywords: carbon dioxide assimilation; electron transport; imaging; metabolism; photosystem II photochemistry; stomata
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 16 Sep 2011 09:03
Last Modified: 06 Jan 2022 13:34

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