Research Repository

Bicuculline-Induced Seizures: A Challenge for Optical and Biochemical Modeling of the Cytochrome Oxidase CuA Nirs Signal

Cooper, Chris E and Cope, Mark and Elwell, Clare E and Delpy, David T (2009) 'Bicuculline-Induced Seizures: A Challenge for Optical and Biochemical Modeling of the Cytochrome Oxidase CuA Nirs Signal.' Advances in Experimental Medicine and Biology, 645. pp. 129-134. ISSN 0065-2598

Full text not available from this repository.


The effect of seizures on brain blood flow and metabolism has been extensively studied. However, few studies have focused on mitochondria. We used near infrared spectroscopy (NIRS) to study hemoglobin and cytochrome oxidase changes during seizures, induced by the GABA antagonist bicuculline, in the adult rat. A broadband spectroscopy system was used with the optodes placed across the rat head. We focused on the initial seizures post-bicuculline addition during which oxyhemoglobin (HbO2) increased, deoxyhemoglobin (HHb) decreased and total hemoglobin (Hbtot) increased. The NIRS signal associated with the oxidised CuA centre of mitochondrial cytochrome c oxidase (oxCCO) decreased. At the highest bicuculline doses (0.25 mg/animal) the maximum values recorded were: ΔHbO2 = +19 ± 7 μM; ΔHHb = -12 ± 4 μM; ΔHbtot = +7 ± 4 μM, ΔoxCCO = - 1.7 ± 0.3 μM. These results are broadly in line with other NIRS studies. However, previous measurements of NADH fluorescence indicate oxidation of the mitochondrial redox chain under these conditions. The changes induced by bicuculline provide an interesting challenge to the physics and biochemistry of using NIRS to study mitochondrial redox states in vivo and we explore the possible spectroscopic and/or biochemical meaning of these apparent anomalies. © Springer Science+Business Media, LLC 2009.

Item Type: Article
Uncontrolled Keywords: Animals; Rats; Rats, Wistar; Seizures; Copper; Bicuculline; Electron Transport Complex IV; Spectroscopy, Near-Infrared; Models, Biological; Male
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 16 Sep 2011 09:03
Last Modified: 15 Jan 2022 00:21

Actions (login required)

View Item View Item