Mason, Maria G and Holladay, Rebecca S and Nicholls, Peter and Shepherd, Mark and Cooper, Chris E (2008) 'A Quantitative Approach to Nitric Oxide Inhibition of Terminal Oxidases of the Respiratory Chain.' GLOBINS AND OTHER NITRIC OXIDE-REACTIVE PROTEINS, PART B, 437. pp. 135-159. ISSN 0076-6879

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Abstract

Inhibition of terminal respiratory oxidases by nitric oxide (NO) plays important physiological roles in signaling and host defense. Using a bacterial quinol oxidase and mitochondrial cytochrome c oxidase, this chapter describes simple polarographic methods to quantify the kinetic characteristics of inhibition by NO. This chapter points out the inherent pitfalls of both experimental design and data analysis and compares alternative methods. Additionally, it describes a system designed to acquire polarographic and spectral data simultaneously to permit identification of spectral intermediates under defined conditions. © 2008 Elsevier Inc. All rights reserved.

Item Type:	Article
Uncontrolled Keywords:	Oxygen; Nitric Oxide; Oxidoreductases; Nitric Oxide Donors; Biosensing Techniques; Inhibitory Concentration 50; Enzyme Activation; Electron Transport; Models, Biological
Subjects:	Q Science > QH Natural history > QH301 Biology
Divisions:	Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of
SWORD Depositor:	Elements
Depositing User:	Elements
Date Deposited:	16 Sep 2011 09:02
Last Modified:	15 Jan 2022 00:21
URI:	http://repository.essex.ac.uk/id/eprint/707

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