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Imaging of Reactive Oxygen Species In vivo

Driever, Steven M and Fryer, Michael J and Mullineaux, Philip M and Baker, Neil R (2009) 'Imaging of Reactive Oxygen Species In vivo.' Methods in molecular biology (Clifton, N.J.), 479. pp. 109-116. ISSN 1064-3745

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Reactive oxygen species (ROS) are involved in many signalling pathways and numerous stress responses in plants. Consequently, it is important to be able to identify and localize ROS in vivo to evaluate their roles in signalling. A number of probes that have a high affinity for specific ROS and that are effectively taken up by cells and tissues are commercially available. Applications to intact leaves of singlet oxygen sensor green (SOSG), nitroblue tetrazolium (NBT), di-amino benzidine (DAB) and Amplex Red to detect singlet oxygen, superoxide and hydrogen peroxide are described. Imaging of the probes in the cells and tissues of leaves allows sites of ROS production to be identified.

Item Type: Article
Uncontrolled Keywords: Arabidopsis; Plant Leaves; Hydrogen Peroxide; Superoxides; Singlet Oxygen; Reactive Oxygen Species
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 15 Sep 2011 12:33
Last Modified: 15 Jan 2022 00:23

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