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The importance of the effect of shear stress on endothelial cells in determining the performance of hemoglobin based oxygen carriers

Gaucher-Di Stasio, C and Paternotte, E and Prin-Mathieu, C and Reeder, BJ and Poitevin, G and Labrude, P and Stoltz, JF and Cooper, CE and Menu, P (2009) 'The importance of the effect of shear stress on endothelial cells in determining the performance of hemoglobin based oxygen carriers.' Biomaterials, 30 (4). 445 - 451. ISSN 0142-9612

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Abstract

The lack of blood donations and the threat of infections from blood and blood products have led to extensive research into the development of blood substitutes. The latest generation of hemoglobin based oxygen carriers (HBOC) has been shown to induce side effects like hypertension, vasoconstriction, inflammation and oxidative stress. HBOC are able to restore volemia and transport oxygen after a hemorrhagic shock, the reperfusion leading to the restoration of the blood flow in vessels. We propose an innovative approach, more closely emulating clinical situations, to assess the impact of HBOC perfusion on endothelial cells (EC) in vitro. Through this approach we quantified levels of oxidative stress, vasoactive factors and inflammation. EC were cultivated under a laminar flow to reproduce the return of shear stress (SS) during the reperfusion. We showed that heme oxygenase I transcription correlated with changes in oxidatively modified heme and methemoglobin; all were lower under SS. SS induced increased nitric oxide production, which may have implications for the mechanism of in vivo vasoconstriction and hypertension. E-selectin changes under SS were greater than those of ICAM-1. Our results demonstrate how it is essential to include SS in assays attempting to understand the potential vascular side effects of HBOC perfusion. © 2008 Elsevier Ltd. All rights reserved.

Item Type: Article
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health > Biological Sciences, School of
Depositing User: Jim Jamieson
Date Deposited: 06 Oct 2011 14:53
Last Modified: 16 Apr 2019 20:15
URI: http://repository.essex.ac.uk/id/eprint/800

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