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Characterisation of the androgen regulation of glycine N-methyltransferase in prostate cancer cells

Ottaviani, Silvia and Brooke, Greg N and O'Hanlon-Brown, Ciara and Waxman, Jonathan and Ali, Simak and Buluwela, Laki (2013) 'Characterisation of the androgen regulation of glycine N-methyltransferase in prostate cancer cells.' Journal of Molecular Endocrinology, 51 (3). pp. 301-312. ISSN 0952-5041

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<jats:p>The development and growth of prostate cancer is dependent on androgens; thus, the identification of androgen-regulated genes in prostate cancer cells is vital for defining the mechanisms of prostate cancer development and progression and developing new markers and targets for prostate cancer treatment. Glycine<jats:italic>N</jats:italic>-methyltransferase (GNMT) is a<jats:italic>S</jats:italic>-adenosylmethionine-dependent methyltransferase that has been recently identified as a novel androgen-regulated gene in prostate cancer cells. Although the importance of this protein in prostate cancer progression has been extensively addressed, little is known about the mechanism of its androgen regulation. Here, we show that GNMT expression is stimulated by androgen in androgen receptor (AR) expressing cells and that the stimulation occurs at the mRNA and protein levels. We have identified an androgen response element within the first exon of the<jats:italic>GNMT</jats:italic>gene and demonstrated that AR binds to this element<jats:italic>in vitro</jats:italic>and<jats:italic>in vivo</jats:italic>. Together, these studies identify GNMT as a direct transcriptional target of the AR. As this is an evolutionarily conserved regulatory element, this highlights androgen regulation as an important feature of GNMT regulation.</jats:p>

Item Type: Article
Uncontrolled Keywords: prostate cancer; androgen receptor; glycine N-methyltransferase; androgen response element
Subjects: R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 04 Dec 2013 10:16
Last Modified: 18 Aug 2022 11:04

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