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Clusters of microRNAs emerge by new hairpins in existing transcripts.

Marco, Antonio and Ninova, Maria and Ronshaugen, Matthew and Griffiths-Jones, Sam (2013) 'Clusters of microRNAs emerge by new hairpins in existing transcripts.' Nucleic Acids Res, 41 (16). 7745 - 7752. ISSN 1362-4962

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Abstract

Genetic linkage may result in the expression of multiple products from a polycistronic transcript, under the control of a single promoter. In animals, protein-coding polycistronic transcripts are rare. However, microRNAs are frequently clustered in the genomes of animals, and these clusters are often transcribed as a single unit. The evolution of microRNA clusters has been the subject of much speculation, and a selective advantage of clusters of functionally related microRNAs is often proposed. However, the origin of microRNA clusters has not been so far explored. Here, we study the evolution of microRNA clusters in Drosophila melanogaster. We observed that the majority of microRNA clusters arose by the de novo formation of new microRNA-like hairpins in existing microRNA transcripts. Some clusters also emerged by tandem duplication of a single microRNA. Comparative genomics show that these clusters are unlikely to split or undergo rearrangements. We did not find any instances of clusters appearing by rearrangement of pre-existing microRNA genes. We propose a model for microRNA cluster evolution in which selection over one of the microRNAs in the cluster interferes with the evolution of the other linked microRNAs. Our analysis suggests that the study of microRNAs and small RNAs must consider linkage associations.

Item Type: Article
Uncontrolled Keywords: Animals, Drosophila melanogaster, Evolution, Molecular, Genome, MicroRNAs
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health > Life Sciences, School of
Depositing User: Antonio Marco
Date Deposited: 08 Nov 2013 10:21
Last Modified: 23 Aug 2019 21:15
URI: http://repository.essex.ac.uk/id/eprint/8314

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