Research Repository

Redox Gene Therapy Protects Human IB-3 Lung Epithelial Cells Against Ionizing Radiation-Induced Apoptosis

Zwacka, Ralf M and Dudus, Lorita and Epperly, Michael W and Greenberger, Joel S and Engelhardt, John F (1998) 'Redox Gene Therapy Protects Human IB-3 Lung Epithelial Cells Against Ionizing Radiation-Induced Apoptosis.' Human Gene Therapy, 9 (9). pp. 1381-1386. ISSN 1043-0342

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Toxicity to nontumor-derived tissue has proven to be a significant obstacle in achieving therapeutic levels of gamma irradiation in the treatment of cancer. The formation of reactive oxygen species (ROS) such as superoxide radicals (O2-) following irradiation is thought to be a major determinant of cellular damage. To this end, we describe the generation of two recombinant adenoviral vectors expressing the radical-scavenging enzymes MnSOD and CuZnSOD to test therapeutic strategies of radioprotection. Using a human lung epithelial cell line (IB-3), we have demonstrated that infections with both Ad.CMVMnSOD or Ad.CMVCuZnSOD significantly increase both the levels of SOD protein and enzymatic activity as compared to control cells. This increase in SOD expression reduced the level of apoptosis at 72 hr post-irradiation by 50% as compared to mock- or Ad.CMVLacZ-infected cells. Such studies provide the foundation for radioprotective gene therapies in the treatment of cancer.

Item Type: Article
Uncontrolled Keywords: Lung; Cell Line; Epithelial Cells; Humans; Adenoviridae; Superoxide Dismutase; Radiation-Protective Agents; Fluorescent Antibody Technique; Blotting, Western; Apoptosis; Transgenes; Genetic Vectors; Radiation, Ionizing; Genetic Therapy
Subjects: R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 11 Jul 2017 16:09
Last Modified: 15 Jan 2022 00:34

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