Research Repository

Prevention of late effects of irradiation lung damage by manganese superoxide dismutase gene therapy

Epperly, M and Bray, J and Kraeger, S and Zwacka, R and Engelhardt, J and Travis, E and Greenberger, J (1998) 'Prevention of late effects of irradiation lung damage by manganese superoxide dismutase gene therapy.' Gene Therapy, 5 (2). pp. 196-208. ISSN 0969-7128

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Organ and tissue damage caused by ionizing irradiation is directly related to volume irradiated, total dose and dose rate. The acute effects are in part mediated by cellular activation of early response genes, including those for transcriptional activators of genes for humoral cytokines. In the lung, as in other organs, recovery from the acute effects of ionizing irradiation does not always correlate with prevention of the critical fate effects, including fibrosis, which contribute to organ failure. An interventional technique by which to protect normal organs from the late effects of irradiation has remained elusive. We now demonstrate that overexpression of a transgene for human manganese superoxide dismutase (MnSOD) delivered by plasmid-liposome, or adenovirus to the lungs of C57BL/6J or Nu/J mice, respectively, before irradiation exposure, decreases the late effects of whole lung irradiation (organizing alveolitis/fibrosis). These data provide a rational basis for the design of gene therapy approaches to organ protection from irradiation damage.

Item Type: Article
Uncontrolled Keywords: Lung; Animals; Mice, Inbred C57BL; Humans; Mice; Mice, Nude; Adenoviridae; Radiation Pneumonitis; Radiation Injuries, Experimental; Superoxide Dismutase; Liposomes; Immunoblotting; Immunohistochemistry; Injections; Polymerase Chain Reaction; Gene Expression; Genetic Vectors; Plasmids; Genetic Therapy
Subjects: R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 17 Jul 2017 08:19
Last Modified: 06 Apr 2022 10:22

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