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Structure and functional motifs of GCR1, the only plant protein with a GPCR fold?

Taddese, Bruck and Upton, Graham J G and Bailey, Gregory R and Jordon, Sian R D and Abdalla, Nuradin Y and Reeves, Philip J and Reynolds, Christopher A (2013) 'Structure and functional motifs of GCR1, the only plant protein with a GPCR fold?' Plant Physiology. ISSN 0032-0889

Plant Physiol.-2013-Taddese-pp.113.228874.pdf

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Whether GPCRs exist in plants is a fundamental biological question. Interest in deorphanizing new G protein coupled receptors (GPCRs), arises because of their importance in signaling. Within plants, this is controversial as genome analysis has identified 56 putative GPCRs, including GCR1 which is reportedly a remote homologue to class A, B and E GPCRs. Of these, GCR2, is not a GPCR; more recently it has been proposed that none are, not even GCR1. We have addressed this disparity between genome analysis and biological evidence through a structural bioinformatics study, involving fold recognition methods, from which only GCR1 emerges as a strong candidate. To further probe GCR1, we have developed a novel helix alignment method, which has been benchmarked against the the class A – class B - class F GPCR alignments. In addition, we have presented a mutually consistent set of alignments of GCR1 homologues to class A, class B and class F GPCRs, and shown that GCR1 is closer to class A and /or class B GPCRs than class A, class B or class F GPCRs are to each other. To further probe GCR1, we have aligned transmembrane helix 3 of GCR1 to each of the 6 GPCR classes. Variability comparisons provide additional evidence that GCR1 homologues have the GPCR fold. From the alignments and a GCR1 comparative model we have identified motifs that are common to GCR1, class A, B and E GPCRs. We discuss the possibilities that emerge from this controversial evidence that GCR1 has a GPCR fold

Item Type: Article
Subjects: Q Science > QA Mathematics
Q Science > QK Botany
Divisions: Faculty of Science and Health > Biological Sciences, School of
Faculty of Science and Health > Mathematical Sciences, Department of
Depositing User: Christopher Reynolds
Date Deposited: 20 Nov 2013 10:26
Last Modified: 16 Dec 2014 11:14

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