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In vivo dendritic cell depletion reduces breeding efficiency, affecting implantation and early placental development in mice

Krey, G and Frank, P and Shaikly, V and Barrientos, G and Cordo-Russo, R and Ringel, F and Moschansky, P and Chernukhin, IV and Metodiev, M and Fernández, N and Klapp, BF and Arck, PC and Blois, SM (2008) 'In vivo dendritic cell depletion reduces breeding efficiency, affecting implantation and early placental development in mice.' Journal of Molecular Medicine, 86 (9). 999 - 1011. ISSN 0946-2716

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Abstract

Implantation of mammalian embryos into their mother's uterus ensures optimal nourishment and protection throughout development. Complex molecular interactions characterize the implantation process, and an optimal synchronization of the components of this embryo-maternal dialogue is crucial for a successful reproductive outcome. In the present study, we investigated the role of dendritic cells (DC) during implantation process using a transgenic mouse system (DTRtg) that allows transient depletion of CD11c+cells in vivo through administration of diphtheria toxin. We observed that DC depletion impairs the implantation process, resulting in a reduced breeding efficiency. Furthermore, the maturity of uterine natural killer cells at dendritic cell knockout (DCKO) implantation sites was affected as well; as demonstrated by decreased perforin expression and reduced numbers of periodic-acid-Schiff (PAS)-positive cells. This was accompanied by disarrangements in decidual vascular development. In the present study, we were also able to identify a novel DC-dependent protein, phosphatidylinositol transfer protein β (PITPβ), involved in implantation and trophoblast development using a proteomic approach. Indeed, DCKO mice exhibited substantial anomalies in placental development, including hypocellularity of the spongiotrophoblast and labyrinthine layers and reduced numbers of trophoblast giant cells. Giant cells also down-regulated their expression of two characteristic markers of trophoblast differentiation, placental lactogen 1 and proliferin. In view of these findings, dendritic cells emerge as possible modulators in the orchestration of events leading to the establishment and maintenance of pregnancy. © 2008 Springer-Verlag.

Item Type: Article
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health > Biological Sciences, School of
Depositing User: Jim Jamieson
Date Deposited: 06 Oct 2011 13:22
Last Modified: 26 Jun 2018 21:15
URI: http://repository.essex.ac.uk/id/eprint/870

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