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Activation of geminivirus V-sense promoters in roots is restricted to nematode feeding sites

ESCOBAR, CAROLINA and GARCÍA, ALEJANDRA and ARISTIZÁBAL, FABIO and PORTILLO, MARY and HERREROS, ESTHER and MUNOZ-MARTÍN, M ANGELES and GRUNDLER, FLORIAN and MULLINEAUX, PHILLIP M and FENOLL, CARMEN (2010) 'Activation of geminivirus V-sense promoters in roots is restricted to nematode feeding sites.' Molecular Plant Pathology, 11 (3). pp. 409-417. ISSN 1464-6722

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Obligate sedentary endoparasitic nematodes, such as the root-knot and cyst nematodes, elicit the differentiation of specialized nematode nurse or feeding cells [nematode feeding sites (NFS), giant cells and syncytia, respectively]. During NFS differentiation, marked changes in cell cycle progression occur, partly similar to those induced by some geminiviruses. In this work, we describe the activation of V-sense promoters from the Maize streak virus (MSV) and Wheat dwarf virus (WDV) in NFS formed by root-knot and cyst nematodes. Both promoters were transiently active in microinjection experiments. In tobacco and Arabidopsis transgenic lines carrying promoter-β-glucuronidase fusions, the MSV V-sense promoter was activated in the vascular tissues of aerial plant parts, primarily leaf and cotyledon phloem tissue and some floral structures. Interestingly, in roots, promoter activation was restricted to syncytia and giant cells tested with four different nematode populations, but undetectable in the rest of the root system. As the activity of the promoter in transgenic rootstocks should be restricted to NFS only, the MSV promoter may have utility in engineering grafted crops for nematode control. Therefore, this study represents a step in the provision of some of the much needed additional data on promoters with restricted activation in NFS useful in biotechnological nematode control strategies. © 2010 The Authors. Journal compilation © 2010 Blackwell Publishing Ltd.

Item Type: Article
Uncontrolled Keywords: Animals; Nematoda; Geminiviridae; Maize streak virus; Plants, Genetically Modified; Arabidopsis; Tobacco; Plant Roots; Glucuronidase; Immunohistochemistry; Microinjections; Feeding Behavior; Gene Expression Regulation, Viral; Promoter Regions, Genetic
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 22 Sep 2011 15:41
Last Modified: 18 Aug 2022 10:58

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