Demidchik, Vadim and Cuin, Tracey A and Svistunenko, Dimitri and Smith, Susan J and Miller, Anthony J and Shabala, Sergey and Sokolik, Anatoliy and Yurin, Vladimir (2010) '<i>Arabidopsis</i> root K+-efflux conductance activated by hydroxyl radicals: single-channel properties, genetic basis and involvement in stress-induced cell death.' Journal of Cell Science, 123 (9). pp. 1468-1479. ISSN 0021-9533

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Abstract

<jats:p>Reactive oxygen species (ROS) are central to plant stress response, signalling, development and a multitude of other processes. In this study, the plasma-membrane hydroxyl radical (HR)-activated K+ channel responsible for K+ efflux from root cells during stress accompanied by ROS generation is characterised. The channel showed 16-pS unitary conductance and was sensitive to Ca2+, tetraethylammonium, Ba2+, Cs+ and free-radical scavengers. The channel was not found in the gork1-1 mutant, which lacks a major plasma-membrane outwardly rectifying K+ channel. In intact Arabidopsis roots, both HRs and stress induced a dramatic K+ efflux that was much smaller in gork1-1 plants. Tests with electron paramagnetic resonance spectroscopy showed that NaCl can stimulate HR generation in roots and this might lead to K+-channel activation. In animals, activation of K+-efflux channels by HRs can trigger programmed cell death (PCD). PCD symptoms in Arabidopsis roots developed much more slowly in gork1-1 and wild-type plants treated with K+-channel blockers or HR scavengers. Therefore, similar to animal counterparts, plant HR-activated K+ channels are also involved in PCD. Overall, this study provides new insight into the regulation of plant cation transport by ROS and demonstrates possible physiological properties of plant HR-activated K+ channels.</jats:p>

Item Type:	Article
Uncontrolled Keywords:	Ion channels; Plant; Potassium; Programmed cell death; Reactive oxygen species; Stress
Subjects:	Q Science > QH Natural history > QH301 Biology
Divisions:	Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of
SWORD Depositor:	Elements
Depositing User:	Elements
Date Deposited:	23 Sep 2011 09:02
Last Modified:	11 Apr 2022 23:51
URI:	http://repository.essex.ac.uk/id/eprint/984

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