Kadiiska, Maria B and Basu, Samar and Brot, Nathan and Cooper, Christopher and Saari Csallany, A and Davies, Michael J and George, Magdalene M and Murray, Dennis M and Jackson Roberts, L and Shigenaga, Mark K and Sohal, Rajindar S and Stocker, Roland and Van Thiel, David H and Wiswedel, Ingrid and Hatch, Gary E and Mason, Ronald P (2013) Biomarkers of oxidative stress study V: Ozone exposure of rats and its effect on lipids, proteins, and DNA in plasma and urine. Free Radical Biology and Medicine, 61. pp. 408-415. DOI https://doi.org/10.1016/j.freeradbiomed.2013.04.023
Kadiiska, Maria B and Basu, Samar and Brot, Nathan and Cooper, Christopher and Saari Csallany, A and Davies, Michael J and George, Magdalene M and Murray, Dennis M and Jackson Roberts, L and Shigenaga, Mark K and Sohal, Rajindar S and Stocker, Roland and Van Thiel, David H and Wiswedel, Ingrid and Hatch, Gary E and Mason, Ronald P (2013) Biomarkers of oxidative stress study V: Ozone exposure of rats and its effect on lipids, proteins, and DNA in plasma and urine. Free Radical Biology and Medicine, 61. pp. 408-415. DOI https://doi.org/10.1016/j.freeradbiomed.2013.04.023
Kadiiska, Maria B and Basu, Samar and Brot, Nathan and Cooper, Christopher and Saari Csallany, A and Davies, Michael J and George, Magdalene M and Murray, Dennis M and Jackson Roberts, L and Shigenaga, Mark K and Sohal, Rajindar S and Stocker, Roland and Van Thiel, David H and Wiswedel, Ingrid and Hatch, Gary E and Mason, Ronald P (2013) Biomarkers of oxidative stress study V: Ozone exposure of rats and its effect on lipids, proteins, and DNA in plasma and urine. Free Radical Biology and Medicine, 61. pp. 408-415. DOI https://doi.org/10.1016/j.freeradbiomed.2013.04.023
Abstract
Ozone exposure effect on free radical-catalyzed oxidation products of lipids, proteins, and DNA in the plasma and urine of rats was studied as a continuation of the international Biomarker of Oxidative Stress Study (BOSS) sponsored by NIEHS/NIH. The goal was to identify a biomarker for ozone-induced oxidative stress and to assess whether inconsistent results often reported in the literature might be due to the limitations of the available methods for measuring the various types of oxidative products. The time- and dose-dependent effects of ozone exposure on rat plasma lipid hydroperoxides, malondialdehyde, F2-isoprostanes, protein carbonyls, methionine oxidation, and tyrosine- and phenylalanine oxidation products, as well as urinary malondialdehyde and F2-isoprostanes were investigated with various techniques. The criterion used to recognize a marker in the model of ozone exposure was that a significant effect could be identified and measured in a biological fluid seen at both doses at more than one time point. No statistically significant differences between the experimental and the control groups at either ozone dose and time point studied could be identified in this study. Tissue samples were not included. Despite all the work accomplished in the BOSS study of ozone, no available product of oxidation in biological fluid has yet met the required criteria of being a biomarker. The current negative findings as a consequence of ozone exposure are of great importance, because they document that in complex systems, as the present in vivo experiment, the assays used may not provide meaningful data of ozone oxidation, especially in human studies. © Published by Elsevier Inc .
Item Type: | Article |
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Uncontrolled Keywords: | Biomarkers; Oxidative stress; Ozone exposure; Rats |
Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 23 Dec 2014 11:10 |
Last Modified: | 07 Aug 2024 18:36 |
URI: | http://repository.essex.ac.uk/id/eprint/10194 |