Hui, Jerome HL and Marco, Antonio and Hunt, Suzanne and Melling, Janet and Griffiths-Jones, Sam and Ronshaugen, Matthew (2013) Structure, evolution and function of the bi-directionally transcribed iab-4/iab-8 microRNA locus in arthropods. Nucleic Acids Research, 41 (5). pp. 3352-3361. DOI https://doi.org/10.1093/nar/gks1445
Hui, Jerome HL and Marco, Antonio and Hunt, Suzanne and Melling, Janet and Griffiths-Jones, Sam and Ronshaugen, Matthew (2013) Structure, evolution and function of the bi-directionally transcribed iab-4/iab-8 microRNA locus in arthropods. Nucleic Acids Research, 41 (5). pp. 3352-3361. DOI https://doi.org/10.1093/nar/gks1445
Hui, Jerome HL and Marco, Antonio and Hunt, Suzanne and Melling, Janet and Griffiths-Jones, Sam and Ronshaugen, Matthew (2013) Structure, evolution and function of the bi-directionally transcribed iab-4/iab-8 microRNA locus in arthropods. Nucleic Acids Research, 41 (5). pp. 3352-3361. DOI https://doi.org/10.1093/nar/gks1445
Abstract
In Drosophila melanogaster, the iab-4/iab-8 locus encodes bi-directionally transcribed microRNAs that regulate the function of flanking Hox transcription factors. We show that bi-directional transcription, temporal and spatial expression patterns and Hox regulatory function of the iab-4/iab-8 locus are conserved between fly and the beetle Tribolium castaneum. Computational predictions suggest iab-4 and iab-8 microRNAs can target common sites, and cell-culture assays confirm that iab-4 and iab-8 function overlaps on Hox target sites in both fly and beetle. However, we observe key differences in the way Hox genes are targeted. For instance, abd-A transcripts are targeted only by iab-8 in Drosophila, whereas both iab-4 and iab-8 bind to Tribolium abd-A. Our evolutionary and functional characterization of a bi-directionally transcribed microRNA establishes the iab-4/iab-8 system as a model for understanding how multiple products from sense and antisense microRNAs target common sites.
Item Type: | Article |
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Uncontrolled Keywords: | Cell Line; Animals; Tribolium; Drosophila melanogaster; MicroRNAs; Chromosome Mapping; Evolution, Molecular; Phylogeny; Transcription, Genetic; Gene Expression Regulation, Developmental; RNA Interference; Genes, Homeobox; Genes, Insect; Genetic Loci |
Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 23 Dec 2014 15:56 |
Last Modified: | 07 Aug 2024 20:02 |
URI: | http://repository.essex.ac.uk/id/eprint/10340 |
Available files
Filename: Nucl. Acids Res.-2013-Hui-3352-61.pdf
Licence: Creative Commons: Attribution-Noncommercial 3.0
Filename: nar-02683-c-2011-File006.pdf