Worrall, Jonathan AR and G贸rna, Maria and Crump, Nicholas T and Phillips, Lara G and Tuck, Alex C and Price, Amanda J and Bavro, Vassiliy N and Luisi, Ben F (2008) Reconstitution and analysis of the multienzyme Escherichia coli RNA degradosome. Journal of Molecular Biology, 382 (4). pp. 870-883. DOI https://doi.org/10.1016/j.jmb.2008.07.059
Worrall, Jonathan AR and G贸rna, Maria and Crump, Nicholas T and Phillips, Lara G and Tuck, Alex C and Price, Amanda J and Bavro, Vassiliy N and Luisi, Ben F (2008) Reconstitution and analysis of the multienzyme Escherichia coli RNA degradosome. Journal of Molecular Biology, 382 (4). pp. 870-883. DOI https://doi.org/10.1016/j.jmb.2008.07.059
Worrall, Jonathan AR and G贸rna, Maria and Crump, Nicholas T and Phillips, Lara G and Tuck, Alex C and Price, Amanda J and Bavro, Vassiliy N and Luisi, Ben F (2008) Reconstitution and analysis of the multienzyme Escherichia coli RNA degradosome. Journal of Molecular Biology, 382 (4). pp. 870-883. DOI https://doi.org/10.1016/j.jmb.2008.07.059
Abstract
The Escherichia coli RNA degradosome is a multienzyme assembly that functions in transcript turnover and maturation of structured RNA precursors. We have developed a procedure to reconstitute the RNA degradosome from recombinant components using modular coexpression vectors. The reconstituted assembly can be purified on a scale that has enabled biochemical and biophysical analyses, and we compare the properties of recombinant and cell-extracted RNA degradosomes. We present evidence that auxiliary protein components can be recruited to the 'superprotomer' core of the assembly through a dynamic equilibrium involving RNA intermediaries. We discuss the implications for the regulation of RNA degradosome function in vivo.
Item Type: | Article |
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Uncontrolled Keywords: | Escherichia coli; Multienzyme Complexes; Endoribonucleases; Phosphopyruvate Hydratase; Polyribonucleotide Nucleotidyltransferase; RNA Helicases; Escherichia coli Proteins; Host Factor 1 Protein; Recombinant Proteins; RNA Precursors; RNA, Bacterial; Nucleic Acid Conformation; Genetic Vectors; DEAD-box RNA Helicases |
Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 11 Oct 2011 11:22 |
Last Modified: | 07 Aug 2024 20:07 |
URI: | http://repository.essex.ac.uk/id/eprint/1078 |