Moon, RW and Hall, J and Rangkuti, F and Ho, YS and Almond, N and Mitchell, GH and Pain, A and Holder, AA and Blackman, MJ (2013) Adaptation of the genetically tractable malaria pathogen Plasmodium knowlesi to continuous culture in human erythrocytes. Proceedings of the National Academy of Sciences of the United States of America, 110 (2). pp. 531-536. DOI https://doi.org/10.1073/pnas.1216457110
Moon, RW and Hall, J and Rangkuti, F and Ho, YS and Almond, N and Mitchell, GH and Pain, A and Holder, AA and Blackman, MJ (2013) Adaptation of the genetically tractable malaria pathogen Plasmodium knowlesi to continuous culture in human erythrocytes. Proceedings of the National Academy of Sciences of the United States of America, 110 (2). pp. 531-536. DOI https://doi.org/10.1073/pnas.1216457110
Moon, RW and Hall, J and Rangkuti, F and Ho, YS and Almond, N and Mitchell, GH and Pain, A and Holder, AA and Blackman, MJ (2013) Adaptation of the genetically tractable malaria pathogen Plasmodium knowlesi to continuous culture in human erythrocytes. Proceedings of the National Academy of Sciences of the United States of America, 110 (2). pp. 531-536. DOI https://doi.org/10.1073/pnas.1216457110
Abstract
Research into the aetiological agent of the most widespread form of severe malaria, Plasmodium falciparum, has benefitted enormously from the ability to culture and genetically manipulate blood-stage forms of the parasite in vitro. However, most malaria outside Africa is caused by a distinct Plasmodium species, Plasmodium vivax, and it has become increasingly apparent that zoonotic infection by the closely related simian parasite Plasmodium knowlesi is a frequent cause of life-threatening malaria in regions of southeast Asia. Neither of these important malarial species can be cultured in human cells in vitro, requiring access to primates with the associated ethical and practical constraints. We report the successful adaptation of P. knowlesi to continuous culture in human erythrocytes. Human-adapted P. knowlesi clones maintain their capacity to replicate in monkey erythrocytes and can be genetically modified with unprecedented efficiency, providing an important and unique model for studying conserved aspects of malarial biology as well as species-specific features of an emerging pathogen.
Item Type: | Article |
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Uncontrolled Keywords: | Erythrocytes; Animals; Macaca fascicularis; Humans; Plasmodium knowlesi; DNA Primers; Cryopreservation; Culture Techniques; Cloning, Molecular; Polymerase Chain Reaction; Sequence Analysis, DNA; Adaptation, Biological; Species Specificity; Base Sequence; Genotype; Molecular Sequence Data |
Subjects: | Q Science > QR Microbiology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 23 Mar 2015 13:16 |
Last Modified: | 16 May 2024 17:53 |
URI: | http://repository.essex.ac.uk/id/eprint/13357 |
Available files
Filename: pnas.201216457.pdf