Crombie, Andrew T and Khawand, Myriam El and Rhodius, Virgil A and Fengler, Kevin A and Miller, Michael C and Whited, Gregg M and McGenity, Terry J and Murrell, J Colin (2015) Regulation of plasmid‐encoded isoprene metabolism in <scp><i>R</i></scp><i>hodococcus</i>, a representative of an important link in the global isoprene cycle. Environmental Microbiology, 17 (9). pp. 3314-3329. DOI https://doi.org/10.1111/1462-2920.12793
Crombie, Andrew T and Khawand, Myriam El and Rhodius, Virgil A and Fengler, Kevin A and Miller, Michael C and Whited, Gregg M and McGenity, Terry J and Murrell, J Colin (2015) Regulation of plasmid‐encoded isoprene metabolism in <scp><i>R</i></scp><i>hodococcus</i>, a representative of an important link in the global isoprene cycle. Environmental Microbiology, 17 (9). pp. 3314-3329. DOI https://doi.org/10.1111/1462-2920.12793
Crombie, Andrew T and Khawand, Myriam El and Rhodius, Virgil A and Fengler, Kevin A and Miller, Michael C and Whited, Gregg M and McGenity, Terry J and Murrell, J Colin (2015) Regulation of plasmid‐encoded isoprene metabolism in <scp><i>R</i></scp><i>hodococcus</i>, a representative of an important link in the global isoprene cycle. Environmental Microbiology, 17 (9). pp. 3314-3329. DOI https://doi.org/10.1111/1462-2920.12793
Abstract
<jats:title>Summary</jats:title><jats:p>Emissions of biogenic volatile organic compounds (<jats:styled-content style="fixed-case">VOCs</jats:styled-content>) form an important part of the global carbon cycle, comprising a significant proportion of net ecosystem productivity. They impact atmospheric chemistry and contribute directly and indirectly to greenhouse gases. Isoprene, emitted largely from plants, comprises one third of total <jats:styled-content style="fixed-case">VOCs</jats:styled-content>, yet in contrast to methane, which is released in similar quantities, we know little of its biodegradation. Here, we report the genome of an isoprene degrading isolate, <jats:styled-content style="fixed-case"><jats:italic>R</jats:italic></jats:styled-content><jats:italic>hodococcus</jats:italic> sp. <jats:styled-content style="fixed-case">AD</jats:styled-content>45, and, using mutagenesis shows that a plasmid‐encoded soluble di‐iron centre isoprene monooxygenase (<jats:styled-content style="fixed-case">IsoMO</jats:styled-content>) is essential for isoprene metabolism. Using <jats:styled-content style="fixed-case">RNA</jats:styled-content> sequencing (<jats:styled-content style="fixed-case">RNAseq</jats:styled-content>) to analyse cells exposed to isoprene or epoxyisoprene in a substrate‐switch time‐course experiment, we show that transcripts from 22 contiguous genes, including those encoding <jats:styled-content style="fixed-case">IsoMO</jats:styled-content>, were highly upregulated, becoming among the most abundant in the cell and comprising over 25% of the entire transcriptome. Analysis of gene transcription in the wild type and an <jats:styled-content style="fixed-case">IsoMO</jats:styled-content>‐disrupted mutant strain showed that epoxyisoprene, or a subsequent product of isoprene metabolism, rather than isoprene itself, was the inducing molecule. We provide a foundation of molecular data for future research on the environmental biological consumption of this important, climate‐active compound.</jats:p>
Item Type: | Article |
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Uncontrolled Keywords: | Rhodococcus; Plants; Pentanes; Butadienes; Hemiterpenes; RNA, Bacterial; Gene Expression Profiling; Sequence Analysis, RNA; Ecosystem; Climate; Base Sequence; Genome, Bacterial; Plasmids; Molecular Sequence Data; Volatile Organic Compounds; Carbon Cycle |
Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QR Microbiology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 15 May 2015 19:06 |
Last Modified: | 07 Aug 2024 19:48 |
URI: | http://repository.essex.ac.uk/id/eprint/13708 |
Available files
Filename: Crombie_et_al-2015-Environmental_Microbiology.pdf
Licence: Creative Commons: Attribution 3.0