Nelson, CJ and Duckney, P and Hawkins, TJ and Deeks, MJ and Laissue, PP and Hussey, PJ and Obara, B (2014) Blobs and curves: object-based colocalisation for plant cells. Functional Plant Biology, 42 (5). pp. 471-485. DOI https://doi.org/10.1071/FP14047
Nelson, CJ and Duckney, P and Hawkins, TJ and Deeks, MJ and Laissue, PP and Hussey, PJ and Obara, B (2014) Blobs and curves: object-based colocalisation for plant cells. Functional Plant Biology, 42 (5). pp. 471-485. DOI https://doi.org/10.1071/FP14047
Nelson, CJ and Duckney, P and Hawkins, TJ and Deeks, MJ and Laissue, PP and Hussey, PJ and Obara, B (2014) Blobs and curves: object-based colocalisation for plant cells. Functional Plant Biology, 42 (5). pp. 471-485. DOI https://doi.org/10.1071/FP14047
Abstract
Blobs and curves occur everywhere in plant bioimaging: from signals of fluorescence-labelled proteins, through cytoskeletal structures, nuclei staining and cell extensions such as root hairs. Here we look at the problem of colocalisation of blobs with blobs (protein-protein colocalisation) and blobs with curves (organelle-cytoskeleton colocalisation). This article demonstrates a clear quantitative alternative to pixel-based colocalisation methods and, using object-based methods, can quantify not only the level of colocalisation but also the distance between objects. Included in this report are computational algorithms, biological experiments and guidance for those looking to increase their use of computationally-based and quantified analysis of bioimages.
Item Type: | Article |
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Uncontrolled Keywords: | actin-binding proteins, bioimage informatics, fluorescence microscopy, image analysis, mitochondrial trafficking, plant science |
Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 09 Jul 2015 10:35 |
Last Modified: | 07 Aug 2024 19:48 |
URI: | http://repository.essex.ac.uk/id/eprint/14084 |