Zakaria, Dalia and Lappin‐Scott, Hilary and Burton, Sara and Whitby, Corinne (2007) Bacterial diversity in soil enrichment cultures amended with 2 (2‐methyl‐4‐chlorophenoxy) propionic acid (mecoprop). Environmental Microbiology, 9 (10). pp. 2575-2587. DOI https://doi.org/10.1111/j.1462-2920.2007.01375.x
Zakaria, Dalia and Lappin‐Scott, Hilary and Burton, Sara and Whitby, Corinne (2007) Bacterial diversity in soil enrichment cultures amended with 2 (2‐methyl‐4‐chlorophenoxy) propionic acid (mecoprop). Environmental Microbiology, 9 (10). pp. 2575-2587. DOI https://doi.org/10.1111/j.1462-2920.2007.01375.x
Zakaria, Dalia and Lappin‐Scott, Hilary and Burton, Sara and Whitby, Corinne (2007) Bacterial diversity in soil enrichment cultures amended with 2 (2‐methyl‐4‐chlorophenoxy) propionic acid (mecoprop). Environmental Microbiology, 9 (10). pp. 2575-2587. DOI https://doi.org/10.1111/j.1462-2920.2007.01375.x
Abstract
<jats:title>Summary</jats:title><jats:p>The <jats:italic>tfdA</jats:italic> gene encodes for an α‐ketoglutarate‐dependent dioxygenase enzyme which catalyses the first step of the degradation of phenoxyalkanoic acid herbicides such as 2 (2‐methyl‐4‐chlorophenoxy) propionic acid (mecoprop). The bacterial diversity of soil enrichment cultures containing mecoprop was examined by Denaturing Gradient Gel Electrophoresis (DGGE) and clone libraries of both 16S rRNA genes and <jats:italic>tfdA</jats:italic> genes. The 16S rRNA gene sequences were diverse and clustered with either the <jats:italic>Beta</jats:italic>‐ or <jats:italic>Gammaproteobacteria</jats:italic>. The 16S rRNA gene sequence from a bacterial strain isolated from an enrichment culture, grown on R‐mecoprop, which represented a dominant band in the DGGE profiles, had a high 16S rRNA sequence identity (100%) to <jats:italic>Burkholderia glathei</jats:italic>. This is the first report that <jats:italic>B. glathei</jats:italic> is implicated in mecoprop degradation. PCR amplification of the <jats:italic>tfdA</jats:italic> genes detected class III <jats:italic>tfdA</jats:italic> genes only, and no class I or class II <jats:italic>tfdA</jats:italic> sequences were detected. To understand the genes involved the degradation of specific mecoprop (R‐) and (S‐) enantiomers, oligonucleotide probes targeting the <jats:italic>tfdA</jats:italic>, <jats:italic>rdpA, sdpA</jats:italic> and <jats:italic>cadA</jats:italic> genes were hybridized to DNA extracted from enrichment cultures grown on either R‐mecoprop or (R/S) racemic mecoprop. Strong hybridization signals were obtained with <jats:italic>sdpA</jats:italic> and <jats:italic>tfdA</jats:italic> probes using DNA extracted from cultures grown on racemic mecoprop. A strong hybridization signal was also obtained with the <jats:italic>rdpA</jats:italic> probe with DNA extracted from the cultures grown on R‐mecoprop. This suggests the <jats:italic>rdpA</jats:italic> gene is involved in R‐mecoprop degradation while <jats:italic>tfdA</jats:italic>, <jats:italic>sdpA</jats:italic> and <jats:italic>cadA</jats:italic> genes are involved in the degradation of both R‐ and S‐mecoprop.</jats:p>
Item Type: | Article |
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Uncontrolled Keywords: | Burkholderia; Betaproteobacteria; Gammaproteobacteria; 2-Methyl-4-chlorophenoxyacetic Acid; Carboxy-Lyases; RNA, Ribosomal, 16S; Herbicides; Soil Microbiology; Phylogeny; Base Sequence; Molecular Sequence Data; Biodegradation, Environmental; Genetic Variation |
Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 18 Jul 2017 08:22 |
Last Modified: | 30 Oct 2024 20:41 |
URI: | http://repository.essex.ac.uk/id/eprint/15839 |