Zakaria, Dalia and Lappin-Scott, Hilary and Burton, Sara and Whitby, Corinne (2007) Bacterial diversity in soil enrichment cultures amended with 2 (2-methyl-4-chlorophenoxy) propionic acid (mecoprop). Environmental Microbiology, 9 (10). pp. 2575-2587. DOI https://doi.org/10.1111/j.1462-2920.2007.01375.x
Zakaria, Dalia and Lappin-Scott, Hilary and Burton, Sara and Whitby, Corinne (2007) Bacterial diversity in soil enrichment cultures amended with 2 (2-methyl-4-chlorophenoxy) propionic acid (mecoprop). Environmental Microbiology, 9 (10). pp. 2575-2587. DOI https://doi.org/10.1111/j.1462-2920.2007.01375.x
Zakaria, Dalia and Lappin-Scott, Hilary and Burton, Sara and Whitby, Corinne (2007) Bacterial diversity in soil enrichment cultures amended with 2 (2-methyl-4-chlorophenoxy) propionic acid (mecoprop). Environmental Microbiology, 9 (10). pp. 2575-2587. DOI https://doi.org/10.1111/j.1462-2920.2007.01375.x
Abstract
The tfdA gene encodes for an α-ketoglutarate-dependent dioxygenase enzyme which catalyses the first step of the degradation of phenoxyalkanoic acid herbicides such as 2 (2-methyl-4-chlorophenoxy) propionic acid (mecoprop). The bacterial diversity of soil enrichment cultures containing mecoprop was examined by Denaturing Gradient Gel Electrophoresis (DGGE) and clone libraries of both 16S rRNA genes and tfdA genes. The 16S rRNA gene sequences were diverse and clustered with either the Beta- or Gammaproteobacteria. The 16S rRNA gene sequence from a bacterial strain isolated from an enrichment culture, grown on R-mecoprop, which represented a dominant band in the DGGE profiles, had a high 16S rRNA sequence identity (100%) to Burkholderia glathei. This is the first report that B. glathei is implicated in mecoprop degradation. PCR amplification of the tfdA genes detected class III tfdA genes only, and no class I or class II tfdA sequences were detected. To understand the genes involved the degradation of specific mecoprop (R-) and (S-) enantiomers, oligonucleotide probes targeting the tfdA, rdpA, sdpA and cadA genes were hybridized to DNA extracted from enrichment cultures grown on either R-mecoprop or (R/S) racemic mecoprop. Strong hybridization signals were obtained with sdpA and tfdA probes using DNA extracted from cultures grown on racemic mecoprop. A strong hybridization signal was also obtained with the rdpA probe with DNA extracted from the cultures grown on R-mecoprop. This suggests the rdpA gene is involved in R-mecoprop degradation while tfdA, sdpA and cadA genes are involved in the degradation of both R- and S-mecoprop. © 2007 The Authors.
Item Type: | Article |
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Uncontrolled Keywords: | Burkholderia; Betaproteobacteria; Gammaproteobacteria; 2-Methyl-4-chlorophenoxyacetic Acid; Carboxy-Lyases; RNA, Ribosomal, 16S; Herbicides; Soil Microbiology; Phylogeny; Base Sequence; Molecular Sequence Data; Biodegradation, Environmental; Genetic Variation |
Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 18 Jul 2017 08:22 |
Last Modified: | 18 Aug 2022 11:33 |
URI: | http://repository.essex.ac.uk/id/eprint/15839 |