Venkatraman, Bhooma and Klenova, Elena (2015) Role of CTCF poly(ADP-Ribosyl)ation in the regulation of apoptosis in breast cancer cells. Indian Journal of Medical and Paediatric Oncology, 36 (01). pp. 49-54. DOI https://doi.org/10.4103/0971-5851.151784
Venkatraman, Bhooma and Klenova, Elena (2015) Role of CTCF poly(ADP-Ribosyl)ation in the regulation of apoptosis in breast cancer cells. Indian Journal of Medical and Paediatric Oncology, 36 (01). pp. 49-54. DOI https://doi.org/10.4103/0971-5851.151784
Venkatraman, Bhooma and Klenova, Elena (2015) Role of CTCF poly(ADP-Ribosyl)ation in the regulation of apoptosis in breast cancer cells. Indian Journal of Medical and Paediatric Oncology, 36 (01). pp. 49-54. DOI https://doi.org/10.4103/0971-5851.151784
Abstract
<jats:title>Abstract</jats:title><jats:p> Introduction: CTCF is a candidate tumor suppressor gene encoding a multifunctional transcription factor. CTCF function is controlled by posttranslational modification and interaction with other proteins. Research findings suggested that CTCF function can be regulated by poly(ADP-ribosyl)ation (PARlation) and has specific anti-apoptotic function in breast cancer cells. The aim of this study is to assess the effect of CTCF-wild type (WT) and CTCF complete mutant, which is deficient of PARlation in regulating apoptosis in breast cancer cells. Materials and Methods: The effect of CTCF-WT and CTCF complete mutant was expressed in breast cancer cell-lines by DNA-mediated transfection technique monitored by enhanced green fluorescent protein fluorescence. Evaluation of apoptotic cell death was carried out with immunohistochemical staining using 4′-6′-diamino-2 phenylindole and scoring by fluorescent microscopy. Results: CTCF-WT supports survival of breast cancer cells and was observed that CTCF complete mutant interferes with the functions of the CTCF-WT and there was a considerable apoptotic cell death in the breast cancer cell lines such as MDA-MB-435, CAMA-1 and MCF-7. Conclusion: The study enlighten CTCF as a "Biological Marker" for breast cancer and the role of CTCF PARlation may be involved in breast carcinogenesis.</jats:p>
Item Type: | Article |
---|---|
Uncontrolled Keywords: | 4-6-diamino-2 phenylindole; CTCF complete mutant; CTCF-wild type; poly(ADP-ribosyl)ation; transfection |
Subjects: | R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 17 Oct 2016 09:23 |
Last Modified: | 07 Aug 2024 17:26 |
URI: | http://repository.essex.ac.uk/id/eprint/17735 |
Available files
Filename: IndianJMedPaediatrOncol36149-1915275_051912.pdf
Licence: Creative Commons: Attribution-Noncommercial-Share Alike 3.0