Kekilli, D and Moreno-Chicano, T and Chaplin, AK and Horrell, S and Dworkowski, FSN and Worrall, JAR and Strange, RW and Hough, MA (2017) Photoreduction and validation of haem?ligand intermediate states in protein crystals by in situ single-crystal spectroscopy and diffraction. IUCrJ, 4 (3). pp. 263-270. DOI https://doi.org/10.1107/S2052252517002159
Kekilli, D and Moreno-Chicano, T and Chaplin, AK and Horrell, S and Dworkowski, FSN and Worrall, JAR and Strange, RW and Hough, MA (2017) Photoreduction and validation of haem?ligand intermediate states in protein crystals by in situ single-crystal spectroscopy and diffraction. IUCrJ, 4 (3). pp. 263-270. DOI https://doi.org/10.1107/S2052252517002159
Kekilli, D and Moreno-Chicano, T and Chaplin, AK and Horrell, S and Dworkowski, FSN and Worrall, JAR and Strange, RW and Hough, MA (2017) Photoreduction and validation of haem?ligand intermediate states in protein crystals by in situ single-crystal spectroscopy and diffraction. IUCrJ, 4 (3). pp. 263-270. DOI https://doi.org/10.1107/S2052252517002159
Abstract
Powerful synergies are available from the combination of multiple methods to study proteins in the crystalline form. Spectroscopies which probe the same region of the crystal from which X-ray crystal structures are determined can give insights into redox, ligand and spin states to complement the information gained from the electron-density maps. The correct assignment of crystal structures to the correct protein redox and ligand states is essential to avoid the misinterpretation of structural data. This is a particular concern for haem proteins, which can occupy a wide range of redox states and are exquisitely sensitive to becoming reduced by solvated electrons generated from interactions of X-rays with water molecules in the crystal. Here, single-crystal spectroscopic fingerprinting has been applied to investigate the laser photoreduction of ferric haem in cytochrome c′. Furthermore, in situ X-ray-driven generation of haem intermediates in crystals of the dye-decolourizing-type peroxidase A (DtpA) from Streptomyces lividans is described.
Item Type: | Article |
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Uncontrolled Keywords: | X-ray photoreduction; laser photoreduction of haem; haem; in crystallo optical spectroscopy |
Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 29 Jun 2017 14:22 |
Last Modified: | 07 Aug 2024 19:56 |
URI: | http://repository.essex.ac.uk/id/eprint/19914 |
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