Eduok, Samuel and Ferguson, Robert MW and Jefferson, Bruce and Villa, Raffaella and Coulon, Frédéric (2017) Aged-engineered nanoparticles effect on sludge anaerobic digestion performance and associated microbial communities. Science of the Total Environment, 609. pp. 232-241. DOI https://doi.org/10.1016/j.scitotenv.2017.07.178
Eduok, Samuel and Ferguson, Robert MW and Jefferson, Bruce and Villa, Raffaella and Coulon, Frédéric (2017) Aged-engineered nanoparticles effect on sludge anaerobic digestion performance and associated microbial communities. Science of the Total Environment, 609. pp. 232-241. DOI https://doi.org/10.1016/j.scitotenv.2017.07.178
Eduok, Samuel and Ferguson, Robert MW and Jefferson, Bruce and Villa, Raffaella and Coulon, Frédéric (2017) Aged-engineered nanoparticles effect on sludge anaerobic digestion performance and associated microbial communities. Science of the Total Environment, 609. pp. 232-241. DOI https://doi.org/10.1016/j.scitotenv.2017.07.178
Abstract
To investigate the potential effect of aged engineered nanoparticles (a-ENPs) on sludge digestion performance, 150L pilot anaerobic digesters (AD) were fed with a blend of primary and waste activated sludge spiked either with a mixture of silver oxide, titanium dioxide and zinc oxide or a mixture of their equivalent bulk metal salts to achieve a target concentration of 250, 2000, and 2800mgkg<sup>-1</sup> dry weight, respectively. Volatile fatty acids (VFA) were 1.2 times higher in the spiked digesters and significantly different (p=0.05) from the control conditions. Specifically, isovaleric acid concentration was 2 times lower in the control digester compared to the spiked digesters, whereas hydrogen sulfide was 2 times lower in the ENPs spiked digester indicating inhibitory effect on sulfate reducing microorganisms. Based on the ether-linked isoprenoids concentration, the total abundance of methanogens was 1.4 times lower in the ENPs spiked digester than in the control and metal salt spiked digesters. Pyrosequencing indicated 80% decrease in abundance and diversity of methanogens in ENPs spiked digester compared to the control digester. Methanosarcina acetivorans and Methanosarcina barkeri were identified as nano-tolerant as their relative abundance increased by a factor of 6 and 11, respectively, compared to the other digesters. The results further provide compelling evidence on the resilience of Fusobacteria, Actinobacteria and the Trojan horse-like effect of ENPs which offered a competitive advantage to some organisms while reducing microbial abundance and diversity.
Item Type: | Article |
---|---|
Uncontrolled Keywords: | Anaerobiosis; Bacteria; Bioreactors; Fatty Acids, Volatile; Metal Nanoparticles; Methane; Methanosarcina; Sewage |
Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 03 Aug 2017 20:46 |
Last Modified: | 07 Aug 2024 19:33 |
URI: | http://repository.essex.ac.uk/id/eprint/20168 |
Available files
Filename: 1-s2.0-S004896971731882X-main.pdf
Licence: Creative Commons: Attribution 3.0
Filename: 1-s2.0-S004896971731882X-mmc1.docx