Teif, VB and Rippe, K (2011) Nucleosome mediated crosstalk between transcription factors at eukaryotic enhancers. Physical Biology, 8 (4). 044001-044001. DOI https://doi.org/10.1088/1478-3975/8/4/044001
Teif, VB and Rippe, K (2011) Nucleosome mediated crosstalk between transcription factors at eukaryotic enhancers. Physical Biology, 8 (4). 044001-044001. DOI https://doi.org/10.1088/1478-3975/8/4/044001
Teif, VB and Rippe, K (2011) Nucleosome mediated crosstalk between transcription factors at eukaryotic enhancers. Physical Biology, 8 (4). 044001-044001. DOI https://doi.org/10.1088/1478-3975/8/4/044001
Abstract
A recent study of transcription regulation in Drosophila embryonic development revealed a complex non-monotonic dependence of gene expression on the distance between binding sites of repressor and activator proteins at the corresponding enhancer cis-regulatory modules (Fakhouri et al 2010 Mol. Syst. Biol. 6 341). The repressor efficiency was high at small separations, low around 30 bp, reached a maximum at 50-60 bp, and decreased at larger distances to the activator binding sites. Here, we propose a straightforward explanation for the distance dependence of repressor activity by considering the effect of the presence of a nucleosome. Using a method that considers partial unwrapping of nucleosomal DNA from the histone octamer core, we calculated the dependence of activator binding on the repressor-activator distance and found a quantitative agreement with the distance dependence reported for the Drosophila enhancer element. In addition, the proposed model offers explanations for other distance-dependent effects at eukaryotic enhancers. © 2011 IOP Publishing Ltd.
Item Type: | Article |
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Uncontrolled Keywords: | Nucleosomes; Animals; Drosophila; Drosophila Proteins; Transcription Factors; Repressor Proteins; Enhancer Elements, Genetic |
Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 16 Oct 2017 10:15 |
Last Modified: | 07 Aug 2024 20:06 |
URI: | http://repository.essex.ac.uk/id/eprint/20477 |