Petrini, Edoardo and Baillet, Victoire and Cridge, Jake and Hogan, Cassandra J and Guillaume, Cindy and Ke, Huiling and Brandetti, Elisa and Walker, Simon and Koohy, Hashem and Spivakov, Mikhail and Varga Weisz, Patrick (2015) A new phosphate-starvation response in fission yeast requires the endocytic function of myosin I. Journal of Cell Science, 128 (20). pp. 3707-3713. DOI https://doi.org/10.1242/jcs.171314
Petrini, Edoardo and Baillet, Victoire and Cridge, Jake and Hogan, Cassandra J and Guillaume, Cindy and Ke, Huiling and Brandetti, Elisa and Walker, Simon and Koohy, Hashem and Spivakov, Mikhail and Varga Weisz, Patrick (2015) A new phosphate-starvation response in fission yeast requires the endocytic function of myosin I. Journal of Cell Science, 128 (20). pp. 3707-3713. DOI https://doi.org/10.1242/jcs.171314
Petrini, Edoardo and Baillet, Victoire and Cridge, Jake and Hogan, Cassandra J and Guillaume, Cindy and Ke, Huiling and Brandetti, Elisa and Walker, Simon and Koohy, Hashem and Spivakov, Mikhail and Varga Weisz, Patrick (2015) A new phosphate-starvation response in fission yeast requires the endocytic function of myosin I. Journal of Cell Science, 128 (20). pp. 3707-3713. DOI https://doi.org/10.1242/jcs.171314
Abstract
Endocytosis is essential for uptake of many substances into the cell, but how it links to nutritional signalling is poorly understood. Here, we show a new role for endocytosis in regulating the response to low phosphate in Schizosaccharomyces pombe. Loss of function of myosin I (Myo1), Sla2/End4 or Arp2, proteins involved in the early steps of endocytosis, led to increased proliferation in low-phosphate medium compared to controls. We show that once cells are deprived of phosphate they undergo a quiescence response that is dependent on the endocytic function of Myo1. Transcriptomic analysis revealed a wide perturbation of gene expression with induction of stress-regulated genes upon phosphate starvation in wild-type but not Δmyo1 cells. Thus, endocytosis plays a pivotal role in mediating the cellular response to nutrients, bridging the external environment and internal molecular functions of the cell.
Item Type: | Article |
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Uncontrolled Keywords: | Schizosaccharomyces; Phosphates; Myosin Heavy Chains; Schizosaccharomyces pombe Proteins; Signal Transduction; Endocytosis; Gene Expression Regulation, Fungal; Gene Deletion; Actin-Related Protein 2; Transcriptome |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 22 Apr 2021 12:11 |
Last Modified: | 07 Aug 2024 20:25 |
URI: | http://repository.essex.ac.uk/id/eprint/21420 |
Available files
Filename: jcs171314.pdf
Licence: Creative Commons: Attribution 3.0