Laissue, PP and Alghamdi, RA and Tomancak, P and Reynaud, EG and Shroff, H (2017) Assessing phototoxicity in live fluorescence imaging. Nature Methods, 14 (7). pp. 657-661. DOI https://doi.org/10.1038/nmeth.4344
Laissue, PP and Alghamdi, RA and Tomancak, P and Reynaud, EG and Shroff, H (2017) Assessing phototoxicity in live fluorescence imaging. Nature Methods, 14 (7). pp. 657-661. DOI https://doi.org/10.1038/nmeth.4344
Laissue, PP and Alghamdi, RA and Tomancak, P and Reynaud, EG and Shroff, H (2017) Assessing phototoxicity in live fluorescence imaging. Nature Methods, 14 (7). pp. 657-661. DOI https://doi.org/10.1038/nmeth.4344
Abstract
Are the answers to biological questions obtained via live fluorescence microscopy substantially affected by phototoxicity? Although a single set of standards for assessing phototoxicity cannot exist owing to the breadth of samples and experimental questions associated with biological imaging, we need quantitative, practical assessments and reporting standards to ensure that imaging has a minimal impact on observed biological processes and sample health. Here we discuss the problem of phototoxicity in biology and suggest guidelines to improve its reporting and assessment.
Item Type: | Article |
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Uncontrolled Keywords: | Vero Cells; Animals; Dermatitis, Phototoxic; DNA Damage; Free Radicals; Microscopy, Fluorescence; Cell Proliferation; Light; Chlorocebus aethiops |
Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 05 Mar 2018 10:22 |
Last Modified: | 07 Aug 2024 20:02 |
URI: | http://repository.essex.ac.uk/id/eprint/21565 |