Fellows, Rachel and Varga Weisz, Patrick (2018) In vitro Enzymatic Assays of Histone Decrotonylation on Recombinant Histones. Bio-protocol, 8 (14). e2924-. DOI https://doi.org/10.21769/bioprotoc.2924
Fellows, Rachel and Varga Weisz, Patrick (2018) In vitro Enzymatic Assays of Histone Decrotonylation on Recombinant Histones. Bio-protocol, 8 (14). e2924-. DOI https://doi.org/10.21769/bioprotoc.2924
Fellows, Rachel and Varga Weisz, Patrick (2018) In vitro Enzymatic Assays of Histone Decrotonylation on Recombinant Histones. Bio-protocol, 8 (14). e2924-. DOI https://doi.org/10.21769/bioprotoc.2924
Abstract
Class I histone deacetylases (HDACs) are efficient histone decrotonylases, broadening the enzymatic spectrum of these important (epi-)genome regulators and drug targets. Here, we describe an in vitro approach to assaying class I HDACs with different acyl-histone substrates, including crotonylated histones and expand this to examine the effect of inhibitors and estimate kinetic constants.
Item Type: | Article |
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Uncontrolled Keywords: | Crotonylation; Acetylation; Butyrylation; Histone deacetylase; Enzyme; Kinetic; Chromatin |
Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 14 Jan 2019 13:15 |
Last Modified: | 07 Aug 2024 15:44 |
URI: | http://repository.essex.ac.uk/id/eprint/23815 |
Available files
Filename: Bio-protocol2924.pdf