Jurado, Jaime and de Navascués, Joaquín and Gorfinkiel, Nicole (2016) α-Catenin stabilises Cadherin–Catenin complexes and modulates actomyosin dynamics to allow pulsatile apical contraction. Journal of Cell Science, 129 (24). pp. 4496-4508. DOI https://doi.org/10.1242/jcs.193268
Jurado, Jaime and de Navascués, Joaquín and Gorfinkiel, Nicole (2016) α-Catenin stabilises Cadherin–Catenin complexes and modulates actomyosin dynamics to allow pulsatile apical contraction. Journal of Cell Science, 129 (24). pp. 4496-4508. DOI https://doi.org/10.1242/jcs.193268
Jurado, Jaime and de Navascués, Joaquín and Gorfinkiel, Nicole (2016) α-Catenin stabilises Cadherin–Catenin complexes and modulates actomyosin dynamics to allow pulsatile apical contraction. Journal of Cell Science, 129 (24). pp. 4496-4508. DOI https://doi.org/10.1242/jcs.193268
Abstract
We have investigated how cell contractility and adhesion are functionally integrated during epithelial morphogenesis. To this end, we have analysed the role of α-Catenin, a key molecule linking E-Cadherin-based adhesion and the actomyosin cytoskeleton, during Drosophila embryonic dorsal closure, by studying a newly developed allelic series. We find that α-Catenin regulates pulsatile apical contraction in the amnioserosa, the main force-generating tissue driving closure of the embryonic epidermis. α-Catenin controls actomyosin dynamics by stabilising and promoting the formation of actomyosin foci, and also stabilises DE-Cadherin (Drosophila E-Cadherin, also known as Shotgun) at the cell membrane, suggesting that medioapical actomyosin contractility regulates junction stability. Furthermore, we uncover a genetic interaction between α-Catenin and Vinculin, and a tension-dependent recruitment of Vinculin to amniosersoa apical cell membranes, suggesting the existence of a mechano-sensitive module operating in this tissue.
Item Type: | Article |
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Uncontrolled Keywords: | alpha-Catenin; DE-Cadherin; Oscillation; Apical contraction; Actomyosin; Vinculin; Morphogenesis |
Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 09 Jul 2019 14:16 |
Last Modified: | 07 Aug 2024 20:27 |
URI: | http://repository.essex.ac.uk/id/eprint/24885 |
Available files
Filename: 4496.full.pdf