Chathoth, Keerthi T and Zabet, Nicolae Radu (2019) Chromatin architecture reorganisation during neuronal cell differentiation in Drosophila genome. Genome Research, 29 (4). pp. 613-625. DOI https://doi.org/10.1101/gr.246710.118
Chathoth, Keerthi T and Zabet, Nicolae Radu (2019) Chromatin architecture reorganisation during neuronal cell differentiation in Drosophila genome. Genome Research, 29 (4). pp. 613-625. DOI https://doi.org/10.1101/gr.246710.118
Chathoth, Keerthi T and Zabet, Nicolae Radu (2019) Chromatin architecture reorganisation during neuronal cell differentiation in Drosophila genome. Genome Research, 29 (4). pp. 613-625. DOI https://doi.org/10.1101/gr.246710.118
Abstract
The organization of the genome into topologically associating domains (TADs) was shown to have a regulatory role in development and cellular functioning, but the mechanism involved in TAD establishment is still unclear. Here, we presented the first high-resolution contact map of Drosophila neuronal cells (BG3) and identified different classes of TADs by comparing this to genome organization in embryonic cells (Kc167). We find that only some TADs are conserved in both cell lines, whereas the rest are cell-specific TADs. This is supported by a change in the enrichment of architectural proteins at TAD borders, with BEAF-32 present in embryonic cells and CTCF in neuronal cells. Furthermore, we observed strong divergent transcription, together with RNA Polymerase II occupancy, and an increase in DNA accessibility at the TAD borders. TAD borders that are specific to neuronal cells are enriched in enhancers controlled by neuronal-specific transcription factors. Our results suggest that TADs are dynamic across developmental stages and reflect the interplay between insulators, transcriptional states and enhancer activities.
Item Type: | Article |
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Uncontrolled Keywords: | Neurons; Cell Line; Chromatin; Animals; Drosophila melanogaster; RNA Polymerase II; DNA-Binding Proteins; Eye Proteins; Drosophila Proteins; Chromatin Assembly and Disassembly; Gene Expression Regulation, Developmental; Protein Binding; Genome, Insect; Neurogenesis; CCCTC-Binding Factor |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 19 Nov 2019 15:57 |
Last Modified: | 07 Aug 2024 18:24 |
URI: | http://repository.essex.ac.uk/id/eprint/25675 |
Available files
Filename: Genome Res.-2019-Chathoth-613-25.pdf
Licence: Creative Commons: Attribution 3.0